银杏叶类黄酮对人胃癌细胞BGC823体外的增殖抑制作用  

Inhibitory effects of Ginkgo biloba leaf flavonoids on proliferation of human gastric cancer cell line BGC823 in vitro

作　　者：张凤[1] 杨桂文[1] 张金凤[1] 安利国[1] 

机构地区：[1]山东师范大学生命科学学院动物抗性重点实验室,山东省济南市250014

出　　处：《世界华人消化杂志》2005年第21期2627-2629,共3页World Chinese Journal of Digestology

摘　　要：目的:提取并测定银杏叶类黄酮(芦丁)的含量;探讨银杏叶类黄酮对体外培养的人胃癌细胞BGC823的增殖抑制作用.方法:乙醇法(700mL/L)提取银杏叶类黄酮;三波长分光光度法测定提取物中类黄酮(芦丁)的含量;MTT法及流式细胞技术观察提取物对人胃癌细胞BGC823的增殖抑制作用.结果:提取物中类黄酮(芦丁)含量为140mg/g.MTT法证实银杏叶类黄酮对人胃癌细胞BGC823增殖有抑制作用,且呈剂量依赖效应.流式细胞术分析表明银杏叶类黄酮将胃癌细胞BGC823的生长周期阻滞于S期,与对照组相比明显增加(42.17±0.50%vs32.13±0.45%,P=0.001),凋亡细胞数与对照组相比也显著增加(4.10±0.03%vs2.21±0.01%,P=0.002).结论:银杏叶类黄酮对人胃癌细胞BGC823增殖有抑制作用,并能阻抑细胞周期进程,诱导细胞凋亡.AIM： To extract the flavonoids from Ginkgo bi/oba leaf, and to investigate its inhibitroy effects on the proliferation of human gastric cancer cell line BGC823 cultured in vitro. METHODS： Ethanol （700 mL/L） was used to extract the flavonoids from the leaf of Ginkgo bi/oba. Three wavelength spectrophotometry was used to determine the content of flavonoids in the extracts. Human gastric cancer cells BGC823 cultured in vitro were treated with different concentrations of the flavonoids, and then the proliferation of the cells was detected by MTT assay and flow cytometry. RESULTS： The content of flavonoids in the extracts was 140 mg/g. The flavonoids from Ginkgo bi/oba leaf inhibited the proliferation of BGC823 cells in a dosedependent manner. The rate of cells in S phase was notably increased as compared with that in the controls （42.17±0.50% vs 32.13±0.45%, P = 0.001 ）, and the apoptotic rate of the cells was also increased （4.10±0.03% vs 2.21±0.01%, P = 0.002）. CONCLUSION： Ginkgo bi/oba leaf flavonoids can inhibit the proliferation of human gastric cancer cell line BGC823 by affecting the cycle the cells.

关 键 词：银杏叶类黄酮 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]