柱前衍生高效液相色谱法测定啤酒中微量甲醛  

Determination of trace formaldehyde in beer by HPLC with precolumn derivatization and UV detection

作　　者：王圣庆[1] 郭瑛[1] 马言顺[1] 龙远德[1] 黄天宝[1] 

机构地区：[1]中国科学院成都有机化学研究所

出　　处：《化学研究与应用》2006年第2期214-216,共3页Chemical Research and Application

摘　　要：甲醛作为消毒剂、工业助剂等已广泛应用于各行各业，也列在食品工业加工助剂推荐名单中。然而，甲醛能引起头痛、恶心，肠胃不舒服、皮肤过敏等反应。美国环保署（EPA）称甲醛可能是致癌诱导物。我国居室内空气中甲醛卫生标准不大于0．08mg／m^3，而饮用水和饮料类中甲醛允许含量在现行的标准中还没有明确的规定。啤酒是人类常用饮品，测定和了解啤酒中甲醛含量很有必要。A reversed phase high performance liquid chromatographic method was described for the determination of trace formaldehyde in beer with precolumn derivatization and UV detection. Formaldehyde in beer was directly derived with acetylacetone reagent to yield 2,6 -diethyl - 3,5 - diacetyl - 1,4 - dihydropyridin（DDL）. DDL was extracted by the reaction solution passing through a Waters OASIS HKLB cartridge and then was analyzed by isocratic HPLC on a Xterra RR18 columa（150×3.9mm I. D. 5/μm） with acetonitrile - water containing 0. 075% TFA（ 1/7 ,v/v）as mobile phase,detection at 254nm and column temperature at 40℃. The Iinearity of the method is satisfactory in the range of 0. 09935 - 9. 935mg/L with the correlation coefficient of 0. 9999. The recoveries range within 96. 1 - 102. 8%. The relative standard deviation is less than 4. 2% and the limit of detection is 0. 4ng.

关 键 词：高效液相色谱法 柱前衍生 啤酒 甲醛 乙酰丙酮 

分 类 号：O657.72[理学—分析化学]