全自动细胞图像分析仪做喉鳞状细胞癌癌细胞核形态学定量分析  

Morphologic quantitative analysis of nucleus in laryngeal squamous cell carcinoma with Automatic Image Cytometry

作　　者：汪涛[1] 黄红彦[2] 崔永华[2] 刘秋润[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院耳鼻咽喉科,上海200011 [2]华中科技大学附属同济医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2006年第3期97-100,104,共5页Journal of Clinical Otorhinolaryngology

摘　　要：目的:定量分析喉鳞状细胞癌(LSCC)癌细胞核的形态,并探讨形态学参数与肿瘤临床的关系。方法:采用全自动细胞图像分析仪对来源于21例LSCC和20例喉部良性病变的83张细胞刷片做核形态定量研究。结果:LSCC癌细胞表现为较大的核面积、平均半径、核周长、核圆球度以及核单位面积DNA含量,其中核面积、核周长、核单位面积DNA含量在肿瘤发生不同部位及不同病理类型间有差异,喉部恶性肿瘤组织中S期细胞细胞核均在77μm2以上,平均核半径和核面积作拟合抛物线(拟合方程Y=aX2+bX+c),提示b值越小(b<0.045),c值越大(c>1.82),则与肿瘤越相关。结论:核形态定量分析认为LSCC癌细胞表现为异常的核形态,结合S期细胞核面积与平均半径和核面积的抛物线拟合方程系数,在辅助细胞学诊断及临床评价肿瘤生物学特性方面意义较大。Objective： Analyze morphological character of nucleus in laryngeal squamous cell carcinoma with Automatic Image Cytometry, discuss the relation between morphological parameters and tumor clinic. Method：We discussed morphological character of nucleus from 83 cytology specimens which included 21 laryngeal squamous cell carcinomas and 20 benign diseases with Automatic Image Cytometry. Result： Nucleus from laryngeal squamous cell carcinomas had bigger nuclear areanuclear radius, nuclear perimeter and more DNA content per nuclear area than from laryngeal benign diseases, furthermore, these parameters showed distinct difference in different anatom- ic positions and different pathologic types. We concluded that nuclear areas of nucleus in S phase from laryngeal squamous cell carcinomas are more than 77μm, Second Order Regression Equation（ Y = aX^2＋bX＋c）described scattered diagrams between mean nuclear radius and nuclear area of laryngeal cytology specimens with different larynx pathology and different cell phase in cell cycle. The more the value of coefficient ＂c＂（c≥l. 82）and the less the value of coefficient ＂b＂ （b〈0. 045）, the more the probability of malignant tumor diagnosis. Conclusion.. Morphologic quantitative analysis parameters, such as nuclear area, mean nuclear radius, nuclear perimeter and DNA content per nuclear area, are suitable for diagnosing laryngeal squamous cell carcinomas. They could offer valuable morphologic quantitative criterion in assisting cytology diagnose and evaluating tumor clinical characters when these parameters associated with nuclear area of nucleus in S-phase and the coefficients of Second Order Regression Equation.

关 键 词：形态定量学 喉肿瘤 癌 鳞状细胞 

分 类 号：R730.267[医药卫生—肿瘤]