β-七叶皂甙钠对大鼠脑出血后炎性损伤的保护作用的研究  

Study of β-aescinate on protective mechanism of inflammatory injury after intracerebral hemorrhage in rats

作　　者：周庆博[1] 李鲁扬[2] 毕建忠[1] 郑广娟[3] 贾青[4] 邵念方[3] 

机构地区：[1]山东大学第二医院,山东济南250033 [2]山东大学齐鲁医院 [3]山东中医药大学 [4]山东医学科学院

出　　处：《中国老年学杂志》2006年第1期72-74,共3页Chinese Journal of Gerontology

基　　金：山东省自然基金课题(Y2002C26)资助

摘　　要：目的探讨β-七叶皂甙钠对脑出血后血浆炎性反应因子损伤的保护作用。方法实验大鼠随机分为假手术组,模型组,七叶皂甙钠组,采用胶原酶Ⅶ诱导大鼠脑出血模型,术后2h七叶皂甙钠组腹腔注射β-七叶皂甙钠(2.8mg/kg),另2组给予等量生理盐水,连续用药(1次/d),分别在给药3d和7d后取血,应用酶联免疫吸附法和放射免疫分析法测定血浆细胞间黏附分子-1(ICAM-1)、肿瘤坏死因子-α(TNF-α)含量。结果与假手术组比较,模型组血浆ICAM-1和TNF-α含量均明显升高(P<0.01),与模型组比较,七叶皂甙钠组血浆ICAM-1和TNF-α含量均明显下降(P<0.01,P<0.05)。结论β-七叶皂甙钠对脑出血后神经细胞损伤具有保护作用,其机制可能与减少ICAM-1和TNF-α的产生,抑制炎症反应有关。Objective To observe the effect of aescinate on inflammatory reactive factors： intercellular adhesion molecular-1 （ ICAM - 1 ） and tumor necrosis factor-α （TNF-α） content in plasma after intracerebral hemorrhage in rats and explore its protective mechanism. Methods Rats were randomly divided into six groups ： sham-operative group, model group, aescinate group. The rat model of intracerebral hemorrhage was induced by collagenase Ⅶ. After 2 hours, β-aescinate was injected intraperitoneally with dose of 2. 8 mg/kg in aescinate group per day, saline was given in the other two groups per day. These rats were killed 3 and 7 days later to detect plasma ICAM-1 and TNF-α levels by fluorospectrophotometry and radioimmunoassay. Results ICAM-1 and TNF-α content in plasma were obviously higher （ P 〈 0. 01 ） in model group than those of sham group （ P 〈 0. 01 ）. ICAM-1 and TNF-α content in plasma were obviously lower （ P 〈 0. 01, P 〈 0. 05 ） in aescinate group than those of model group. Conclusions α-aescinate has an evident protective effect on injured neuron after intracerebral hemorrhage in rats, which may be related to decreasing ICAM-1, TNF-α content in plasma and inhabiting inflammatory reaction.

关 键 词：七叶素 脑出血 细胞间黏附分子-1 肿瘤坏死因子-Α 

分 类 号：R743.34[医药卫生—神经病学与精神病学]