人早幼粒白血病HL60细胞及其亚系中mdr－1基因和癌基因表达的关系  

Correlation between expression of mdr 1 gene and oncogenes in human promyelocytic leukemic HL60 cell line and sublines 1

作　　者：周卫东[1] 张鸿卿[1] 方敏 薛绍白[1] 

机构地区：[1]北京师范大学生物系

出　　处：《中国药理学报》1996年第5期471-473,共3页Acta Pharmacologica Sinica

摘　　要：人早幼粒白血病ＨＬ６０细胞及其亚系中ｍｄｒ１基因和癌基因表达的关系周卫东，张鸿卿，方敏，薛绍白（北京师范大学生物系，北京１００８７５，中国）关键词癌基因；多种抗药性；流式细胞计量术；急性早幼粒白血病目的：研究人早幼粒白血病ＨＬ６０细胞系及其分别抗Ｈ...IM: To study the relationship between expression of oncogenes and multiple drug resistant (MDR) phenotype. METHODS: The drug resistant level of HL60 cell line and its sublines were determined with flow cytometry. RNA Dot blot hybridization was used to identify the expression of oncogenes and mdr 1 gene. RESULTS: The expression of mdr 1 gene was in the opposite relation with c myc expression, but in the positive relation with c H ras gene expression in the multiple drug resistant cell lines. In non MDR cell line HL60/RA, the expression levels of mdr 1, c myc , and c H ras were the same as HL60 parental cells. CONCLUSION: Multiple drug resistance is related to not only mdr 1 expression, but also some oncogenes expression level.

关 键 词：癌基因 多种抗药性 白血病 HL60 表达 

分 类 号：R733.703[医药卫生—肿瘤] R915[医药卫生—临床医学]