烟曲霉产壳聚糖酶液体发酵条件研究  

Study on conditions of chitosanase from Aspergillus fumigatus by liquid fermentation

作　　者：周桂[1] 何子平[2] 禤金彩[1] 

机构地区：[1]广西民族大学化学与生态工程学院,广西南宁530006 [2]广西农业科学院,广西南宁530007

出　　处：《海洋科学》2006年第2期34-37,共4页Marine Sciences

基　　金：广西壮族自治区青年基金项目(桂科青0481036);广西壮族自治区教育厅资助项目(桂教科研2002-316)

摘　　要：以烟曲霉(Aspergillus fumigatus)为出发菌,通过选择适合的培养条件,筛选出产壳聚糖酶的菌株。实验结果表明,烟曲霉是良好的产壳聚糖酶出发菌。烟曲霉液体发酵产壳聚糖酶最适培养基组成为:壳聚糖1%,KH2PO40.06%,尿素0.2%,(NH4)2SO40.1%,MgSO40.012%,20%土豆汁3%,NaCl 0.05%。在pH 5.0、温度28℃、摇床转速120 r/min培养条件下振荡培养3 d,烟曲霉产壳聚糖酶的能力最强,达25.1 U/mL。A mutant of Aspergillus fumigatus, which could synthesize chitosanase effectively under the optimized conditions, was obtained with selective nutrient mediums. The optimized liquid fermentation mediums were as follows： chitosan 1%,KH2PO40.06%, Urea 0.2%,（NH4）2SO40. 1%,MgSO40. 012%, 20%potato juice 3%,NaCl0.05%, and pH5.0. After 3 days＇ fermentation at 120 r/min and 28℃, the chitosanase activity produced by the mutant of A. fumigatus reached a higher level of 25.1 U/mL.

关 键 词：烟曲霉(Aspergillus fumigatus) 壳聚糖酶 液体发酵 

分 类 号：Q55[生物学—生物化学]