Genistein对人卵巢癌细胞系TC-1增殖抑制和诱导凋亡作用的研究  

Effects of genistein on proliferation inhibition and apoptosis induction in human ovarian carcinoma cell lines TC-1 .

作　　者：田爽[1] 辛晓燕[1] 黄艳红[1] 胡学昱[2] 袁鹏[1] 张潍[1] 

机构地区：[1]第四军医大学西京医院妇产科,西安710032 [2]第四军医大学西京医院全军骨科研究所,西安710032

出　　处：《现代妇产科进展》2006年第1期28-31,F0003,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(30472226)

摘　　要：目的:研究植物雌激素染料木黄酮(genistein)对人卵巢癌细胞系TC-1细胞增殖的抑制作用和凋亡的诱导作用,探讨其抗癌作用的机制。方法:应用MTT法检测不同浓度genistein对TC-1细胞的生长抑制作用,电镜观察药物作用后细胞超微结构的改变,流式细胞仪检测细胞周期、定量分析凋亡,DNA琼脂糖凝胶电泳法确定凋亡。结果:TC-1细胞经不同浓度genistein处理后,细胞生长明显受到抑制,且这种抑制作用呈时间及浓度依赖性,5mg/L和10mg/L genistein作用72h后,抑制率分别达到89·84%和97·99%。genistein阻断TC-1细胞生长于G2/M期,在G0/G1前出现典型的亚二倍体凋亡峰,且凋亡呈时间依赖性。电镜观察到用药后凋亡细胞典型的形态学特征。DNA凝胶电泳可观察到细胞凋亡特异的DNA梯形带。结论:genistein对TC-1细胞生长的抑制作用呈明显的时间及浓度依赖性,并诱导其发生凋亡。Objective ：To investigate the effects of genistein on proliferation inhibition and apoptosis induction in human ovarian carcinoma cell lines TC-1. Methods ：After culturing with different dose of genistein for different time, the cells were tested by MTF assay. Study on the uhrastructure of TC-1 cells was performed after the administration of genistein by transmission electron microscope （TEM）. Apoptosis rate and cell cycle phase distribution of TC-1 cells were measured by flow cytometry （FCM）. Apoptosis was confirmed by agarose gel electrophoresis （AGE） of DNA. Results ：After TC-1 cells were treated with different concentration of genistein ,dose and time dependent growth inhibition was demonstrated. Inhibition percentage in cells exposed to 5. 0mg/L and 10mg/L genistein for 72 hours were 89.84% and 97.99% respectively. Also, genistein could block TC-1 cells in the G2/M phase of cell cycle. The characteristic morphological changes of apoptosis in genistein treated TC-1 cells were observed by TEM. A typical DNA ladder pattern appeared on AGE. Conclusion ： Genistein can inhibit growth and induce apoptosis in ovarian carcinoma cell lines TC-1 in dose and time dependent modelity.

关 键 词：染料木黄酮 卵巢肿瘤 人卵巢癌细胞系TC-1 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]