人胃癌细胞中NF-κB和VEGF的定量表达  

Expression of Nuclear Factor of Kappa B and Vascular Endothelial Growth Factor in Human Gastric Cancer

作　　者：王菊岩[1] 刘舒颖[1] 吴康英[1] 陈立军[1] 

机构地区：[1]广东医学院附属医院消化内科消化内镜中心,广东湛江524001

出　　处：《海南医学》2006年第3期11-12,129,共3页Hainan Medical Journal

摘　　要：目的探讨核转录因子(nuclearfactorofkappaB,NF-κB)和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)在人胃癌细胞中表达及其与临床病理特征的相关性。方法利用荧光定量聚合酶链反应法测定32例人胃癌细胞和25例癌旁细胞内NF-κBmRNA和VEGFmRNA的定量表达,分析其与临床病理特征的相关性。结果人胃癌细胞中NF-κBmRNA和VEGFmRNA的表达与性别、年龄无关(P>0.05)。人胃癌细胞中NF-κBmRNA和VEGFmRNA的表达明显高于癌旁细胞的表达(P<0.01)。人胃癌细胞中NF-κBmRNA和VEGFmRNA的表达与组织类别、浸润深度、分化程度、淋巴结转移、分期和大小有关(P<0.01)。人胃癌细胞中NF-κBmRNA与VEGFmRNA呈无显著相关性(r=0.2196,P=0.2271)。结论NF-κBmRNA表达上调可能与人胃癌发生、发展和转移有关。Objective to determine expression of nuclear factor of kappa B and vascular endothelial growth factor, and its relation to chnicopathological characteristics in human gastric cancer. Methods Expression of NF-κB mRNA and YEGF mRNA in 32 cases gastric cancer and their adjacent noncancerous tissues was measured by fluorescent quantitative polymerase chain reaction（FQ-PCR）. Results Expression of NF-κB mRNA and VEGF mRNA was not correlated with sex, age in human gastric cancer（P〉0.05）. Expression of NF-κB mRNA and VEGF mRNA in human gastric cancer was significantly higher than that in their adjacent noncancerous tissues（P〈0.01）. Expression of NF-κB mRNA and VEGF mRNA was correlated with histological grades, depth of tumor invasion, tumor differentiation, lymph node metastasis and tumor size in human gastric cancer（P〈0.01）. No significant correlation in human gastric cancer were found between expression of NF-κB mRNA and VEGF mRNA（r=0.2196, P=0.2271）. Conclusions Our results show that overexpression of NF-κB mRNA and VEGF mRNA could play an important role in the development, process and metastasis of human gastric cancer.

关 键 词：胃肿瘤 NF-ΚB 血管内皮生长因子 荧光定量聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]