姜黄素与氟尿嘧啶抗人结肠癌细胞株HT-29增殖的协调作用研究  

THE SYNERGISTIC ANTI-PROLIFERATIVE EFFECT OF CURCUMIN AND FLUOROURACIL ON HUMAN COLON CANCER CELL LINE HT-29

作　　者：杜伯雨[1] 姜丽平[2] 夏泉[3] 卢永科[1] 陈新志[1] 仲来福[1] 

机构地区：[1]大连医科大学毒理研究室 [2]大连医科大学中日合作医药研究所 [3]大连医科大学生物化学与分子生物学教研室,大连116027

出　　处：《营养学报》2006年第1期75-78,共4页Acta Nutrimenta Sinica

摘　　要：目的:检测姜黄素能否增强氟尿嘧啶(fluorouracil,FU)抗结肠癌细胞的增殖作用,并探讨其与环氧合酶-2(cyclooxygenase-2,COX-2)蛋白表达的关系。方法:应用中位效应原理和联合作用指数法来定量姜黄素与FU的联合作用。应用Westernblot分析联合应用两种药物之后对于COX-2蛋白表达的影响。结果:姜黄素和FU的半数生长抑制率(IC50)分别为15.9±1.96和17.3±1.85μmol/L。当姜黄素与FU联合应用时,在较高的作用水平可以定量地观察到对HT-29细胞的协同抑制作用。并且这种协同作用随COX-2蛋白表达水平下调。结论:姜黄素可以增强FU的化学治疗效果,在结肠癌的临床治疗方面联合应用这两种药物可能具有实际意义。Objective ： To demonstrate whether the suppression of cyclooxygenase-2 （COX-2） expression by curcumin can increase the anti-colon-cancer effectiveness of fluorouracil （FU）. Method. The combination effect was quantitatively determined by using the method of median-effect principle and the combination index. The COX-2 expression inhibition after treatment of the combination of curcumin and FU was also evaluated by Western blot analysis. Results： The IC50 values in the HT-29 cells for curcumin and FU were 15.9±1.96, and 17.3±1.85 μmol/L respectively. When curcumin and FU were jointly used, the synergistic inhibition of growth was quantitatively demonstrated at higher effect levels. And this synergism was associated with decreased expression of COX-2 protein. Conclusion： Curcumin can enhance the chemotherapy effect of FU, and this combined treatment may have clinical implications in the treatment of colon cancer. [ACTA NUTRIMENTA SINICA, 2006, 28（1）： 75-78]

关 键 词：姜黄素 氟尿嘧啶 联合作用 环氧合酶-2 

分 类 号：R735.35[医药卫生—肿瘤]