卵巢原发癌与转移癌中Survivin mRNA和Caspase-3 mRNA的临床研究  

Expression of survivin in ovary epithelial carcinoma and its correlation with expression of caspase-3

作　　者：宋和存[1] 李慧[1] 赵杨[1] 

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110001

出　　处：《肿瘤防治杂志》2005年第24期1891-1894,共4页China Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨凋亡抑基因Survivin mRNA和凋亡终末效应器Caspase-3mRNA在卵巢原发癌和转移癌发病机制中的作用及相关关系。方法:应用原位杂交技术检测10例卵巢正常组织、16例卵巢良性肿瘤、38例卵巢原发癌和36例卵巢转移癌组织中Survivin mRNA和Caspase-3mRNA的基因表达,分析其表达的阳性率、相关性以及与临床病理的关系。结果:Survivin mRNA在正常卵巢组织和卵巢良性肿瘤、卵巢原发癌、卵巢转移癌表达中的阳性率分别为3·8%、60·5%和75·0%。Caspase-3mRNA在卵巢正常组织和良性肿瘤、卵巢原发癌和转移癌表达中的阳性率为56·2%、28·7%和27·8%。统计分析显示,survivin mRNA和Caspase-3mRNA的表达在卵巢恶性肿瘤(原发癌和转移癌)与卵巢正常组织、卵巢良性肿瘤中的阳性比率差异有统计学意义,P=0·000,P=0·025。两种基因在卵巢原发癌和卵巢转移癌之间的表达差异无统计学意义,χ2=1·767,P=0·184;χ2=0·012,P=0·911。在卵巢原发癌中的表达与临床分期有关,与年龄、组织分化程度和有无淋巴结转移无关。分析显示SurvivinmRNA与Caspase-3mRNA呈负相关关系,r=-0·434,P<0·01。结论:Survivin mRNA在卵巢原发癌和转移癌中高表达,与卵巢临床分期关系密切,是重要的预后因子。与Caspase-3mRNA的负相关关系提示survivin mRNA在肿瘤凋亡过程其承担重要的作用。OBJECTIVE： To investigate the expression of survivin and its correlation with expression of Caspase-3 in ovarian epithelial carcinoma. METHODS： The expressions of survivin and Caspase-3 were determined by in situ hybridization in 10 normal ovary tissues, 16 benign tumors of ovary, 38 primary ovarian cancer tissues and 36 metastatic ovarian cancer tissues. The expression of survivin and its correlation with the expression of Caspase-3 and clinical data of ovarian carcinoma were analysed by statistics. RESULTS： The positive rates of survivn mRNA in normal ovary tissues and benign tumor of ovary, primary, metastatic epithelial ovarian cancer were 3.8%, 60. 5% and 75. 0%. The positive rates of Caspase-3 mRNA were 56.2%, 28.7% and 27. 8% in normal ovary tissues, benign tumor of ovary, primary and metastatic epithelial ovarian cancer. The statistics suggested that the expressions of survivin and Caspase-3 were significantly different among normal ovary tissue, benign tumor, primary and metastatic epithelial ovarian cancer, P=0. 000; P=0. 025. The expressions of two pieces of gene had no statistical significance X^2 = 1. 767, P = 0. 184; X^2 = 0. 012, P = 0. 911. The positive expression of the two pieces of gene was associated with clinical stage and long term survival, except for age, histological grade and lymph node metastasis. Survivin was negatively associated with Caspase-3, r = - 0. 434, P〈 0. 01. CONCLUSIONS： The expression of survivin mRNA is highly expression in primary and metastatic epithelial ovarian cancer. Survivin is closely associated with clinical stage and is an important prognosis factor. The negative correlation between the expressions of survivin and Caspase-3 suggests survivin may play an important role in tumor apoptosis.

关 键 词：癌 原位 卵巢肿瘤/病理学 肿瘤 多原发性 肿瘤转移 

分 类 号：R737.31[医药卫生—肿瘤]