蝎毒抗癌多肽对小鼠骨髓抑制的造血和免疫功能恢复的影响  

The Effects of An Antineoplastic Polypeptide(APⅢ1) from Buthus Martensii Venom on Hemopoietic and Immune Recovery in Myelosuppressive Mice

作　　者：王丽娜[1] 董晓先[1] 何慧华[1] 王桂房[1] 卢康荣[1] 李华[1] 孔天翰[2] 董伟华[1] 

机构地区：[1]广州医学院病理生理学教研室,广东广州510182 [2]广州医学院蛇毒研究所,广东广州510182

出　　处：《广州医学院学报》2005年第5期14-16,共3页Academic Journal of Guangzhou Medical College

基　　金：广东省科技攻关项目(2003A3080302);广州市生物毒素重点实验室资助项目(穗科要字[2005]6号)

摘　　要：目的:研究蝎毒抗癌多肽(APⅢ1)对小鼠化疗后致骨髓抑制造血和免疫细胞恢复的影响。方法:应用造血祖细胞培养、流式细胞术等方法,观察APⅢ1对环磷酰胺(CTX)化疗后第14天小鼠骨髓粒单系集落形成单位(CFU-GM)以及CD34、CD117(c-k it)、CD3、CD19等抗原阳性细胞数量的影响,并进行骨髓有核细胞计数和观察小鼠的生存状况。结果:与化疗模型组相比,APⅢ1治疗组的骨髓有核细胞数、CFU-GM集落数、CD34、CD117(c-k it)、CD3、CD19等抗原阳性细胞数量均显著升高,小鼠生存状况有所改善。结论:蝎毒抗癌多肽(APⅢ1)能促进小鼠化疗后致骨髓抑制造血和免疫细胞数量的恢复。Objective： To evaluate the effect of antineoplastic polypeptide component from Buthus martensii venom （APⅢ1 ） on the hemopoietic and immune recovery in myelosappresive mice induced by chemotherapy. Methods： On the day 14 ＇after chemotherapy, the ability of CFU-GM clone forming and the number of cells which express the antigen of CD34, CDll7 （c-kit） , CD3, CD19, etc, were studied by means of the culture of HPC and flow cytometry. The number of nucleated cells in bone marrow was counted and the survival period of the mice observed. Results： Compared with the chemotherapy group, the number of bone marrow nucleated cells, CFU-GM, and the cells which express the antigen of CD34, CD117 （c-kit） , CD3, CD19 ,etc, were all increased in the APⅢ 1 group, and the status of the mice was improved in the AP Ⅲ1 group. Conclusion ： AP Ⅲ 1 can increase the number of haematopoietic and immune cells in mice＇ s bone marrow after chemotherapy indnced myelosuppressive.

关 键 词：骨髓抑制 蝎毒抗癌多肽(APⅢ1) 造血 免疫 功能 

分 类 号：R73-362[医药卫生—肿瘤]