应用RNAi技术沉默STAT3基因对乳腺癌MCF-7细胞的影响  

Inhibition of MCF-7 breast cancer cell by applying RNAi to silence STAT3 gene

作　　者：王禹[1] 张颖超[1] 潘玉琢[2] 赵雪俭[2] 关文曾[1] 

机构地区：[1]吉林大学第二医院基本外科,长春130041 [2]吉林大学基础医学院病理生理教研室,长春130021

出　　处：《免疫学杂志》2006年第1期31-33,共3页Immunological Journal

基　　金：长春市科委基金资助项目(04-07SF020)

摘　　要：目的探讨应用RNAi技术沉默信号转导子与转录活化子3(STAT3)基因对乳腺癌MCF-7细胞的影响。方法应用RNAi技术,以psilencer1.0-U6-STAT3-siRNA重组质粒体外转染MCF-7细胞,采用MTT实验、流式细胞仪检测MCF-7转染前后细胞增殖、细胞周期和早期凋亡的变化,通过Western blotting及半定量RT-PCR检测STAT3基因不同水平的表达。结果MTT实验、流式细胞仪的结果显示,重组质粒转染组的MCF-7细胞的增殖明显受到抑制,且出现凋亡现象;Western blot-ting及半定量RT-PCR结果显示,重组质粒转染组细胞的STAT3基因表达在蛋白及RNA水平上都显著低于对照组(P<0.01),而空白对照组及空质粒组无明显差异(P>0.05)。结论应用RNAi技术沉默STAT3基因可以降低乳腺癌MCF-7细胞STAT3的表达,进而抑制细胞的生长、增殖及诱导细胞的凋亡。Objective To investigate the inhibition of human breast cancer MCF-7 cell by applying RNAi technique to silence signal transducers and activators of transcription 3 （STAT3） gene. Methods MCF-7 cells were transfected with recombinant plasmid psilencerl. 0-U6- STAT3-siRNA by using RNAi technique. The changes of proliferation, cell cycle, and apoptosis was examined by flow cytometry and MTT. The mRNA and protein expressious of STAT3 in cells were determined by Western blotting and RT- PCR, respectively. Results The growth and proliferation of MCF-7 cell was obviously inhibited and the apoptosis of MCF-7 cell was appeared. The mRNA and protein expressious of STAT3 in transfected cells were significantly lower than those of untransfected cells （P 〈 0.01 ）, but there were no significant difference between blank control group and empty plasmid control group （ P 〉 0.05 ）. Conclusion Silencing STAT3 gene by the RNAi technology can decrease the expression of STAT3 gene, suppress the growth and proliferation of MCF-7 cell, and induce apoptosis of MCF-7 cell.

关 键 词：乳腺癌 信号转导子与转录活化子3 RNA干涉 

分 类 号：R737.9[医药卫生—肿瘤]