膀胱移行细胞癌HPV-16/18感染与FHIT基因改变的相关性研究  

Correlation analysis between HPV-16/18 infection and abnormalities of FHIT gene in transitional cell carcinoma of the urinary bladder

作　　者：郑闪[1] 肖泽均[2] 陈汶[3] 邸雪冰[1] 何祖根[4] 高燕宁[1] 

机构地区：[1]中国协和医科大学中国医学科学院肿瘤医院肿瘤研究所病因及癌变研究室,北京100021 [2]中国协和医科大学中国医学科学院肿瘤医院肿瘤研究所泌尿外科 [3]中国协和医科大学中国医学科学院肿瘤医院肿瘤研究所流行病学研究室 [4]中国协和医科大学中国医学科学院肿瘤医院肿瘤研究所病理科

出　　处：《临床泌尿外科杂志》2006年第1期23-25,28,共4页Journal of Clinical Urology

基　　金：国家自然科学基金(39370291);北京市自然科学基金资助项目(7012027);首都医学发展科研基金(2002~2014)资助

摘　　要：目的:探讨膀胱移行细胞癌(TCC)肿瘤组织中HPV-16/18感染与FHIT基因改变的关系。方法:根据HPV-16/18E7DNA检测结果,将32例膀胱TCC病例分为HPV-16/18感染阳性和阴性两组。选择位于FHIT基因第5内含子内的两个微卫星多态标记(D3S1234和D3S1300),对上述两组膀胱TCC患者的肿瘤组织DNA进行杂合性缺失(LOH)分析;同时,对相应的组织病理切片进行FHIT蛋白的免疫组化染色。所得用SPSS10.0软件行统计学处理。结果:在32例膀胱TCC患者的肿瘤组织中,上述两个微卫星多态标记中至少有一个发生LOH的频率为56.25%(18/32);FHIT蛋白表达下调率达84.38%(27/32)。统计学处理未发现HPV感染与FHIT基因改变相关。结论:FHIT基因LOH和FHIT蛋白表达下调是膀胱TCC中的常见异常改变,而HPV-16/18感染可能通过FHIT基因改变以外的其他途径参与膀胱TCC的发生过程。Objective：To investigate the correlation between HPV-16/18 infection and the genetic abnormalities of FHIT gene in the transitional cell carcinoma （TCC）. Methods：Based on the results of detecting DNA of HPV 16/18 E7. 32 cases of TCC were divided into HPV-positive and -negative group. Microsatellite analysis was ap plied using the two pairs of ,primer that amplify the polymorpbic sequences. D3S1234 and D3S1300, located in the intron 5 of the FHIT gene. Immunohistochemical staining was also performed to detect the expression of the FHIT protein in the tumor tissues. SPSS10.0 soflware was used in statistics analysis to process the investigating data. Results：For one or both of the loci. the frequencies of loss of heterozygosity （LOH） detected in the tumor tissues from the 32 TCC patients were 56.25% （18/ 32）. Down regulated expression of FHIT protein in the tumor tissues was found in 27 out of the 32 （81.38%） cnses. There was no obvious correlation between HPV 16/18 infection and the alteration of FHIT gene （ P 〉0.05）.Conclusions：LOH of FHIT gene and down-regulation of FHIT protein were the usual abnormalities in TCC. HPV-16 18 infection may facilitate carcinogenesis of the transitional epithelium of urinary bladder via other mechanism besides the aheration of the FHIT.

关 键 词：膀胱肿瘤 移行细胞癌 肿瘤标记物 

分 类 号：R737.11[医药卫生—肿瘤]