人工合成免疫刺激CpG DNA联合鸡新城疫疫苗对SPF鸡免疫应答的影响  

Effect of Different Concentration of CpG Motifs on Immune Responses of SPF Chicken

作　　者：田兴山[1] 张玲华[1] 周风珍[1] 

机构地区：[1]广东省农业科学院农业生物技术研究所,广东广州510640

出　　处：《中国兽医学报》2006年第1期14-16,共3页Chinese Journal of Veterinary Science

基　　金：广州市科技攻关重点项目(2003Z2-E0221);广东省农业攻关项目(2003B21403)

摘　　要：以鸡新城疫病毒为模式病毒,分别以不同浓度人工合成的免疫刺激序列CpG DNA联合鸡新城疫低毒力活疫苗免疫SPF新生小鸡,通过ELISA法检测鸡血清中抗-NDV抗体水平,并用MTT法检测淋巴细胞白介素-2诱生活性以及淋巴细胞增殖指数(SI),结果显示不同浓度的CpG DNA均能显著提高仔鸡的特异性抗体滴度、淋巴细胞白介素-2诱生活性以及淋巴细胞增殖反应,证明CpG DNA能显著增强鸡对常规疫苗的免疫应答能力。The newborn chicken were immunized by the different concentration of CpG motifs and Newcastle disease mild living vaccine ,the titre of specific antibodies to Newcastle disease mild living vaccine were measured by ELISA ,the proliferation of lymphocytes and induced IL-2 activities were detected by MTT. The results showed that the above-mentioned immune responses of the newborn chicken to different concentration of CpG motifs were significantly stronger than routine immunities.

关 键 词：CPG 鸡新城疫低毒力活疫苗 免疫应答 

分 类 号：S852.52[农业科学—基础兽医学]