肝细胞癌端粒酶活性及hTERT mRNA表达与术后早期复发的关系  

Correlation of postoperative early recurrence with both telomerase activity and human telomerase reverse transcriptase mRNA expression in hepatocellular carcinoma

作　　者：范瑞芳[1] 卫立辛[1] 沈锋[1] 王维峰[1] 贾凤岐[1] 卜欣欣[1] 郭献灵[1] 吴孟超[1] 

机构地区：[1]第二军医大学东方肝胆外科医院肿瘤免疫和基因治疗中心,上海200438

出　　处：《肿瘤》2006年第1期48-51,共4页Tumor

基　　金：国家自然科学基金项目(编号:30471994);国家"九七三"项目(编号:G2000057001);上海市科委基础研究重大项目(编号:04DZ14006);上海市科委浦江人才计划项目(编号:05PJ1410)

摘　　要：目的研究肝细胞癌端粒酶活性及人端粒酶逆转录酶(hTERT)mRNA表达与肝细胞癌术后早期复发的关系。方法采用ELISA-TRAP法检测60例肝癌组织及其癌旁组织端粒酶活性,RT-PCR法检测hTERT mRNA表达,5例正常肝脏组织作为对照。分析端粒酶活性及hTERT mRNA表达与临床病理之间的关系。结果肝癌组织端粒酶活性及hTERTmRNA表达阳性率分别为86.7%(52/60)及90%(54/60),癌旁组织端粒酶活性及hTERT mRNA表达阳性率分别为40%(24/60)及43.3%(26/60)。正常肝脏组织均未检测到端粒酶活性及hTERT mRNA表达。癌旁组织端粒酶活性及hTERTmRNA表达与术后早期复发及包膜浸润、门静脉侵犯、肝内转移等恶性肿瘤的恶性生物学行为有关。结论癌旁组织端粒酶活性及hTERT mRNA表达可能是肝细胞癌术后早期复发的预后指标。Objective：To study the correlation of postoperative early recurrence with both telomerase activity and human telomerase reverse transcriptase （hTERT） mRNA expression in hepatocellular carcinoma （HCC）. Methods： Telomerase activity was detected by ELISA-based telomeric repeat amplification protocol （ELISA-TRAP） in 60 HCC tissues and adjacent non-tumor liver tissues at a distance of 2 cm from HCC nodules. Expression of hTERT mRNA was measured by reverse transcriptional polymerase chain reaction （RT-PCR）. Five normal liver tissues were used as control. The correlation of clinicopathological features with both telomerase activity and hTERT mRNA expression was analyzed statistically. Results：The positive rates of telomerase activity and hTERT mRNA expression were 86.7% （52/60） and 90%（54/60）, respectively, in HCC tissues, and 40%（24/ 60） and 43.3M （26/60）, respectively, in adjacent non-tumor liver tissues. Neither telomerase activity nor hTERT mRNA expression was detected in 5 normal liver tissues. Telomerase activity and hTERT mRNA expression in adjacent non-tumor liver tissues were closely associated with the postoperative early recurrence and the biologically malignant behaviors of the tumor itself such as infiltration of inflammatory cells around fibrous capsule, portal venous invasion, and intrahepatic metastasis. Couclusious：Telomerase activity and hTERT mRNA expression in adjacent non-tumor liver tissues of HCC may serve as useful predictors of postoperative early recurrence.

关 键 词：肝肿瘤 癌 肝细胞 端粒酶 人端粒酶逆转录酶 肿瘤复发 

分 类 号：R735.7[医药卫生—肿瘤]