胃癌腹膜转移中GALECTIN-3MRNA的表达与意义  

Expression of Galectin-3 mRNA in Gastric Cancer with Peritoneal Metastasis

作　　者：杨志明[1] 伍晓汀[1] 何涛[1] 达明绪[1] 罗婷[1] 钱昆[1] 

机构地区：[1]四川大学华西医院普外科

出　　处：《四川大学学报（医学版）》2006年第1期105-108,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家教育部重点项目基金(项目编号02056)资助

摘　　要：目的探讨GALECTIN-3基因在胃癌腹膜转移中的作用。方法通过实时定量RT-PCR方法,对35例同期伴腹膜转移病人的胃癌原发灶、淋巴结转移灶、腹膜转移灶、正常胃粘膜组织中GALECTIN-3MRNA的表达水平进行实时定量RT-PCR检测。结果胃癌原发灶(385.639±98.534)、腹膜转移灶(368.589±93.431)、淋巴结转移灶(375.920±94.346)中GALECTIN-3MRNA表达量同正常胃粘膜(2.158±0.896)中的表达量相比,差异具有统计学意义(P<0.05),胃癌原发灶、腹膜转移灶及淋巴结转移灶三者中的表达量两两相比,差异无统计学意义(P>0.05)。GALECTIN-3MRNA在胃癌原发灶与腹膜转移灶中的表达呈正相关(R=0.997,P=0.000)。结论GALECTIN-3MRNA在胃癌中的高表达与胃癌腹膜转移密切相关。胃癌病灶的GALECTIN-3MRNA检测可作为术前判断胃癌已伴腹膜转移或具有腹膜转移潜能的生物学标记。Objective To search for the role of galectin-3 gene in gastric cancer with peritoneal metastasis. Methods Using real-time quantitative RT-PCR for detecting the expression of galectin-3 mRNA in 35 matched samples, including primary cancer lesions, lymph node lesions, peritoneal metastasis and adjacent noncancerous mucosa. ANOVA and SNK-q test were used. Results The levels of mean expression of galectin-3 mRNA in gastric cancer （385. 639 ± 98. 534）, in peritoneal metastasis （368. 589 ± 93. 431） and in lymph node metastasis （375. 920 ± 94. 346） were significantly higher than that in noncancerous mucosa （2. 158 ± 0. 896） ; there were statistically significant differences respectively（P〈0. 05）. However, the levels of the expression in gastric cancer, in peritoneal metastasis and in lymph node metastasis were shown to be of no significant differences by multiple comparison, P〈0. 05. The expression level in the group of gastric cancer was noted to have positive correlation with that in the group of peritoneal metastasis. These data indicated that the expression levels of galectin-3 in gastric cancer were well correlated to peritoneal metastasis. Conclusion The overexpression of galectin-3 in gastric cancer is well correlated with peritoneal metastasis. Galectin-3 mRNA level may serve as a biological marker in the pre-operative judgment on whether the gastric cancer has had peritoneal metastasis or has just the potential of metastasis.

关 键 词：胃癌 腹膜转移 GALECTIN-3 实时定量RT-PCR 

分 类 号：R735.2[医药卫生—肿瘤]