应用蛋白质组技术对维甲酸耐药相关蛋白的初步分析  

Proteomic Analysis of Proteins Related to Retinoid Acid Resistance

作　　者：秦慧[1] 刘霆[1] 杨金亮[2] 杨寒朔[2] 孟文彤[1] 阚兵[2] 魏于全[2] 赵霞[3] 柳斌[2] 宋鑫[2] 

机构地区：[1]四川大学华西医院血液科,成都610041 [2]四川大学华西医院人类疾病生物治疗教育部重点实验室 [3]四川大学华西第二医院妇产科

出　　处：《四川大学学报（医学版）》2006年第1期133-136,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家863计划(批准号2002AA230011);国家自然科学基金(批准号30200296)资助

摘　　要：目的探讨采用蛋白质组学技术研究维甲酸的耐药机制。方法提取维甲酸敏感细胞株NB4及维甲酸耐药细胞株MR2的总蛋白,进行双向凝胶电泳识别差异表达蛋白,经质谱分析、数据库搜索进行蛋白鉴定。结果单张凝胶电泳图上呈现约600多个蛋白点,组间的匹配性较好,初步筛选出三个表达差异蛋白,经鉴定分别为DJ-1蛋白、热休克蛋白70及KIAA1289未知功能蛋白。结论应用双向电泳连接质谱的蛋白质组学方法有助于筛选到耐药相关的表达蛋白,为进一步研究维甲酸耐药机制提供分子基础。Objective To evaluate the applicability of proteomic methods for shedding light on the mechanisms of All-trans retinoic acid resistance. Methods The expression of cellular proteins in the retinoid acid sensitive cell line NB4 and retinoid acid resistant cell line MR2 was analyzed using the two-dimensional polyacrylamide gel electrophoresis （2D-PAGE）. Differentially expressed proteins were analyzed by mass spectrometry for peptide mass finger and identified by SWISS-PROT protein database. Results Approximately 600 spots appeared in an sodium dod （SDS-GEL）. The match of the expression of cellular proteins between NB4 and MR2 was better. Three significantly differentially expressed proteins were screened and identified to DJ-1 and HSP70, as well as KIAA1289 of which the function is not yet known. Conclusion The utilization of 2D-PAGE, coupled with mass spectrometry is effective in screening the drug resistance-associated proteins and could provide molecular targets for the elucidation of resistance mechanisms.

关 键 词：蛋白质组学 双向凝胶电泳 维甲酸耐药相关蛋白 

分 类 号：R96[医药卫生—药理学]