信号转导子和转录激活子6及其mRNA在哮喘大鼠气道炎症中的作用  

Effect of signal transducer and activator of transcription 6 and its mRNA on airway inflammation in a rat asthma model

作　　者：叶乐平[1] 李昌崇[1] 李绍波[1] 范小芳[2] 胡晓光[1] 张维溪[1] 李孟荣[1] 

机构地区：[1]温州医学院附属儿童医院呼吸科 [2]温州医学院肺心病研究室,浙江温州325027

出　　处：《中国病理生理杂志》2006年第1期107-111,共5页Chinese Journal of Pathophysiology

基　　金：浙江省自然科学基金(No.301485)资助项目;中华儿科杂志百利儿科科研基金资助项目(2003年)

摘　　要：目的:探讨信号转导子和转录激活子6(STAT6)及其mRNA在哮喘大鼠气道炎症中的作用。方法:20只二级雄性SD大鼠随机分为2组,对照组和哮喘组。以卵清白蛋白(OVA)致敏激发法复制大鼠哮喘模型,每只大鼠左肺留取肺组织,右肺进行支气管肺泡灌洗并留取支气管肺泡灌洗液(BALF)。对BALF进行细胞总数、嗜酸性粒细胞(EOS)计数和分类计数;应用双抗体夹心酶联免疫吸附试验(SandwichELISA)法测定BALF和血清中IL-4浓度;采用免疫组化法和原位杂交法测定STAT6蛋白和STAT6mRNA的表达情况。结果:(1)BALF和血清中白细胞介素4(IL-4)的浓度哮喘组均显著高于对照组(均P<0.01);(2)免疫组化和原位杂交显示,哮喘组支气管STAT6蛋白和STAT6mRNA的表达均显著高于对照组(均P<0.01),其主要表达细胞是上皮细胞;(3)支气管上皮细胞STAT6蛋白及其mRNA含量分别与BALF中的IL-4浓度、EOS绝对值呈非常显著正相关。结论:哮喘大鼠STAT6蛋白和mRNA高表达,上皮细胞是其主要表达细胞,并与IL-4浓度、EOS募集密切相关。AIM： To study the effect of signal transducer and activator of transcription 6 （STAT6） on airway inflammation of rats with asthma. METHODS： Male Sprague - Dawley rats were randomly divided into control group and asthma group. The lung tissue was sampled from the left lung. Bronchoalveolar lavage fluid （BALF） was collected from the right lung. The concentrations of IL - 4 in serum and BALF were measured by sandwich ELISA. STAT6 protein and STAT6 mRNA were observed in the epithelial cells of bronchus by the technique of immunohistochemistry and in situ hybridization. RESULTS： （1） The concentrations of IL - 4 in BALF and serum of asthma group were significantly higher than those in control group （ P 〈 0.01 ）. （2） STAT6 protein and STAT6 mRNA in the epithelial cells of bronchus of asthma group were significantly higher than those in control group （ P 〈 0.01 ） ; （3） There was a significant correlation between the concentration of IL- 4 in BALF and STAT6 protein, STAT6 mRNA, respectively. There was a significant correlation between EOS count in BALF and STAT6 protein, STAT6 mRNA respectively in the epithelial cells of bronchus. CONCLUSION： SYAT6 protein and mRNA are expressed in the rat with asthma, the epithelial cells are the chief expression cells, and STAT6 expression has correlation with the concentration of IL- 4 and EOS gathering.

关 键 词：哮喘 信号转导子和转录激活子6 大鼠 上皮细胞 

分 类 号：R363[医药卫生—病理学]