清热解毒与养阴增液法对内毒素脱毒相关分子HDL和ALP影响的研究  

Effects of strategies of heat-clearing and detoxifying, and Yin-invigorating and fluid-supplementing on HDL and ALP molecules associated with endotoxin-degrading

作　　者：王开富[1] 陆付耳[1] 徐丽君[1] 杨明炜[1] 邹欣[1] 李鸣真[1] 叶望云[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北武汉430030

出　　处：《中国病理生理杂志》2006年第1期135-138,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.39870925)

摘　　要：目的:探讨清热解毒和养阴增液法对内毒素脱毒相关分子高密度脂蛋白(HDL)、碱性磷酸酶(ALP)的影响。方法:给家兔耳缘静脉注射大肠杆菌内毒素复制内毒素血症模型,以清热解毒、养阴增液两种温病治法制剂进行治疗,观察各组动物血清白细胞介素1(IL-1)、肿瘤坏死因子-α(TNF-α)、HDL、ALP水平变化及肝、肾组织中ALPmRNA的表达水平。结果:清热解毒组血清IL-1、TNF-α水平显著低于模型组,伴随血清ALP活性及肝肾组织中ALPmRNA表达明显强于模型组;养阴增液组血清IL-1、TNF-α水平也明显低于模型组,而伴随的是血浆HDL水平明显高于模型组。结论:清热解毒和养阴增液两种温病治法制剂均能拮抗内毒素所致IL-1、TNF-α等炎性细胞因子的过度释放,但两者对内毒素脱毒相关分子的影响有别,前者可能主要通过促进ALP活性升高和上调肝肾组织ALPmRNA的表达而起作用,后者可能与提高血浆HDL水平有关。AIM： To explore the therapeutic strategies of heat- clearing and detoxifying （HCDT） and Yin- invigorating and fluid- supplementing （YIFS）, the method of Chinese medicine, on the high density lipeprotein （HDL） and alkaline phosphatase （ALP） molecules, which are associated with endotoxin-degradation. METHODS： The animal model of endotoxemia was estabhshed by the injection of E. Coli lipepelysaccharides through rabbits ear vein. The endotoxemic rabbits were treated respectively with two representative herbal preparations of therapeutic principles against febrile diseases： HCDT or YIFS preparations. The serum levels of interleukin - 1 （ IL - 1 ）, tumor necrosis factor α （TNF -α） and HDL, the ALP activity, the expression of ALP mR- NA in liver and kidney tissues were observed. RESULTS. The serum levels of IL - 1 and TNF -α in HCDT group were significantly decreased, while the serum ALP activity and the expression of ALP mRNA in liver and kidney tissues were obviously increased, as compared with those in model group. Meanwhile, the serum levels of IL- 1 and TNF-α in YIFS group were significantly reduced, and its plasma HDL level was elevated. CONCLUSIONS： Both the herbal preparations, HCDT and YIFS, have the scavenging effects on the overproduction of inflammatory cytokines such as IL - 1 and TNF - α induced by endotoxin, but their effects on the endotoxin- degrading molecules might be different. HCDT principally increases ALP activity and enhances ALP expression in liver and kidney tissues, while YIFS might preferably facihtate the elevation of plasma HDL level.

关 键 词：中草药 脂蛋白类 HDL 碱性磷酸酶 白细胞介素1 肿瘤坏死因子 

分 类 号：R363[医药卫生—病理学]