糖基化终产物对人单核细胞源树突状细胞糖基化终产物受体表达的研究  

Effect of advanced glycosylation end products on the expression of receptor for advanced glycosylation end products in human monocyte-derived dendritic cells

作　　者：贾庆哲[1] 葛均波[1] 梁春[2] 罗育坤[1] 黄东[1] 王克强[1] 陈灏珠[1] 

机构地区：[1]上海市心血管病研究所 [2]第二军医大学长征医院心内科,上海200003

出　　处：《中国病理生理杂志》2006年第1期139-142,共4页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展规划资助项目(973)(No.G2000056903);上海市科委资助项目(No.02JC14026)

摘　　要：目的:探讨糖基化终产物(AGEs))对人单核细胞源树突状细胞(MDCs)糖基化终产物受体(RAGE)表达的影响。方法:用免疫磁珠分离人外周血CD14+单核细胞,经含rhGM-CSF(100μg/L)和rhIL-4(50μg/L)的RPMI-1640培养,使其分化为MDCs,采用RT-PCR和Westernblotting法,观察糖基化-白蛋白(AGE-BSA)对MDCsRAGEmRNA和蛋白表达的影响,同时检测培养液上清中IFN-γ和IL-12的浓度。结果:AGE-BSA诱导DCsRAGEmRNA和蛋白的表达(P<0.05),高于空白对照组,并且明显促进了DCsIFN-γ和IL-12的分泌(P<0.05)。BSA干预组与空白对照组相比差异无显著(P>0.05)。结论:AGEs能够上调DCsRAGE的表达,并且促进了DCsIFN-γ和IL-12的分泌,这可能是糖尿病通过DCs促进动脉粥样硬化发生的重要机制之一。AIM： To investigate the effect of advanced glycosylation end products on the expression of receptor for advanced glycosylation end products in human monocytederived dendritic cells. METHODS： Monocytes were purified （over 98% ） using anti- CD14^＋ microbeads. After 8 d culture in RPMI- 1640 medium containing rhGM- CSF （100 μg/L） and rhIL- 4 （50 μg/L）, immature MDCs were derived, then exposed to AGE - BSA （0 or 200 mg/L） for 24 h. Expression of RAGE was semi - quantified by RT- PCR and Western blotting. At the same time, supematants were collected. IFN- γ and IL- 12 were analyzed by ELISA. RESULTS： mRNA and protein of RAGE incubated by 200 mg/L AGE - BSA was higher than that in control at 24 h. Treatment of DCs with AGE- BSA resulted in about two- fold increase in the expression of RAGE （ P 〈 0.05）. The concentrations of IFN- γ and IL- 12 were both significantly higher than that in control （ P 〈0.05）. CONCLUSION： AGEs up- regulates the expression of RAGE and induces the secretion of IFN -γ and IL - 12 by DCs. These findings may provide insight into the effect of DCs on the processes of atherosclerosis.

关 键 词：树突细胞 糖基化终产物 动脉硬化 单核细胞 

分 类 号：R363[医药卫生—病理学]