表达LacZ基因的重组CVI988病毒的构建及特性研究  

Construction and Characterization of Recombinant Marek's Disease Virus CVI988 Expressing the E.coli LacZ Gene

作　　者：邱亚峰[1] 葛菲菲[1] 张雪莲[1] 徐学清[1] 陈溥言[1] 

机构地区：[1]南京农业大学动物疫病诊断与免疫重点实验室,江苏南京210095

出　　处：《中国病毒学》2006年第1期71-74,共4页Virologica Sinica

基　　金：国家863高新技术发展项目(2002AA245051)

摘　　要：The LacZ gene controlled under the Marek’s disease virus(MDV)glycoprotein B(gB)promoter was excised from plasmid pSK-gB-LacZ and inserted into US10 gene.Two plasmids were constructed,pUS-gB-LacZ(L)and pUS-gB-LacZ(U)),which differ with regard to the orientation of the expression cassette.Then pUS-gB-LacZ(L)was transfected into secondary Chicken embryo fibroblasts(secondary CEF)cells and super-infected with the MDV CVI988 strain.The recombinant virus(rCVILacZ)expressing the lacZ gene was isolated and purified in secondary CEF.The growth curve of rCVILacZ was similar to that of the parent virus in CEF.The LacZ gene controlled under the Marek＇s disease virus （MDV） glycoprotein B （gB） promoter was excised from plasmid pSK-gB-LacZ and inserted into US10 gene. Two plasmids were constructed, pUS-gB-LacZ （L） and pUS-gB-LacZ（U））,which differ with regard to the orientation of the expression cassette. Then pUS-gB-LacZ （L） was transfected into secondary Chicken embryo fibroblasts （secondary CEF） cells and super-infected with the MDV CVI988 strain. The recombinant virus （rCVILacZ） expressing the lacZ gene was isolated and purified in secondary CEF. The growth curve of rCVILacZ was similar to that of the parent virus in CEF.

关 键 词：重组病毒 马立克氏病毒 LACZ基因 启动子 

分 类 号：Q78[生物学—分子生物学]