液基细胞学在体表肿瘤诊断中的应用  

The application of ThinPrep cytology in the diagnosis of superficial tumors

作　　者：季洪爱[1] 王靖华[2] 周晓军[1] 吉耘[1] 刘红娟[1] 吴慰霖[1] 祁正茂[1] 

机构地区：[1]南京军区南京总医院病理科,江苏南京210002 [2]南京军区南京总医院肿瘤科,江苏南京210002

出　　处：《医学研究生学报》2006年第1期69-70,73,共3页Journal of Medical Postgraduates

摘　　要：目的:在体表肿瘤细针吸取细胞学(FNAC)诊断中对应用液基薄层细胞学检查(TCT)的优越性进行评价。方法:运用细针(外径7mm)穿刺抽吸标本,部分标本涂制成常规涂片,立即固定于95%乙醇溶液10m in后,行苏木精-伊红(H-E)染色,光镜检查。将针头内残余的标本迅速注入清洗液,离心沉淀后再转移至有新柏氏细胞保存液的瓶中,经处理后制成薄片数张,95%乙醇固定,1张行H-E染色,光镜观察;其余涂片则用于免疫细胞化学染色。结果:84例体表肿块经TCT和常规FNAC涂片比较,诊断敏感性基本相等;特异性均为100%。TCT固定标本速度快,细胞不易变形,分布均匀、背景清晰。在FNAC检查涂片中,肿瘤细胞易堆积,有时细胞有变性,背景不清晰。免疫细胞化学染色TCT明显优于常规FNAC涂片。结论:应用新技术TCT对肿瘤标本进行诊断,同时辅以常规FNAC检查和免疫细胞化学技术等多种方法检测,其诊断结果的准确性、可靠性有明显提高。Objective： To investigate the advantage of ThinPrep cytological test in tumor diagnosis by fine needle aspiration cytology. Methods ： Using fine needle aspiration for smears, some of smears were made in conventional smears, the remains aspirating needles were washed with a transport washing solution. ThinPrep slides were made using the ThinPrep Processor. Results： In 84 cases FNA samples, 49 cases were breast, 29 cases were lymph node and 6 cases were others. Comparing with conventionally prepared specimens, ThinPrep processed samples showed the advantage in sensitivity or specificity. The lack of cellular debris, blood and exudates improve the ability to detect cellular abnormalities andspeed up the time required for screening in ThinPrep processed samples. Rapid cell transfer from the sampling device to the transporing fluid containing the fixative ensure preservation of cell details with minimal artifactual changes. Conclusion ： The use of Thinprep and by combination of FNAC and immunocytochemistry cytopathological diagnosis can be improved significantly.

关 键 词：液基薄层细胞学检查 肿瘤 细针吸取细胞学 免疫细胞化学 

分 类 号：R730.43[医药卫生—肿瘤]