As_2O_3碘油栓塞对兔VX_2肝癌凋亡、增殖及肝功能的影响  

Effect on Apoptosis and Proliferation of Tumor Cells,and Liver Function of Rabbits after TACE Treatment with As_2O_3 and Lipiodol

作　　者：吴文娟[1] 邹英华[2] 南月敏[3] 李海涛[1] 刘向东[1] 杨春[4] 崔慧先[4] 

机构地区：[1]河北医科大学第三医院放射科,河北石家庄050051 [2]北京大学第一医院医学影像科 [3]河北医科大学第三医院中西医结合肝病科 [4]河北医科大学解剖教研室

出　　处：《实用放射学杂志》2006年第1期1-4,共4页Journal of Practical Radiology

基　　金：河北省卫生厅科研基金资助课题(编号:04019)

摘　　要：目的观察肝动脉As2O3碘油栓塞对兔肝移植瘤凋亡、增生细胞核抗原(PCNA)表达及肝功能的影响。方法40只家兔肝内肿瘤种植后2周,随机分为5组,经肝动脉插管分别给予不同处理,实验设生理盐水灌注组(A组)、As2O3灌注组(B组)、单纯碘油栓塞组(C组)、阿霉素碘油栓塞组(D组)及As2O3+碘油栓塞组(E组),As2O3的用量为2 mg/kg。治疗前3 d,治疗后4、7 d,耳缘静脉取血,测定部分肝功能指标。采用原位末端标记法检测肿瘤细胞的凋亡指数,免疫组化方法测定肿瘤细胞增生细胞核抗原的表达。结果治疗后4 d,栓塞治疗组AST、ALT上升,治疗后7 d,肝功能渐趋正常,阿霉素(ADM)碘油栓塞治疗组AST、ALT水平高于其它组。各组的凋亡指数和增殖指数分别为1.53±0.42、1.82±0.41、2.66±0.54、2.91±0.32、3.44±0.65和60.8±15.5、55.9±14.8、42.4±11.2、40.6±8.8、28.5±5.7,两者存在负相关。结论As2O3比ADM对正常肝组织的毒性低。As2O3碘油栓塞治疗后残余肿瘤的凋亡增加,肿瘤细胞的增殖能力下降。Objective To investigate the effect on apoptotic index, expression of proliferating cell nuclear antigen （PCNA） in hepatic tumor tissue and the effect on liver function after TACE with As2O3 and Lipiodol. Methods Forty New Zealand white rabbits after implanted VX2 hepatic tumor 2 weeks were randomly divided into five groups and the treatments were performed via the hepatic artery with different methods . The level of AST and ALT in serum was respectively tested at 3 days before treatmeat , 4 days and 7 days after treatment . The apoptosis index and proliferation index in tumor tissue were examined one week after treatment using TUNEL （in situ nick end-labeling） and anti-PCNA monoclonal antibody,respectively. Results The level of AST and ALT increased at 4 days after embolization treatment. 7 days after treatment, the level of AST and ALT tended to normal, in ADM treatment group, the AST and ALT level was higher than that in other groups. Apoptosis index （AI）oftumors was 1.53±0.42 , 1.82±0.41 , 2.66±0.54 , 2.91±0.32 and 3.44±0.65, Proliferation index （ PI ） was 60.8±15.5, 55.9-14.8, 42.4±11.2, 40.6 ±8.8 and 28.5 ±5.7 in group A,B, C, D and E, respectively, there was negative correlation between AI and PI . Conclusion The toxicity of As2O3 to normal liver tissue is lower than that of ADM. The apoptosis of residual tumor cells is increased and the proliferation is decreased in As2O3 treatment group.

关 键 词：肝肿瘤 家兔 VX2 肿瘤 凋亡 介入放射学 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]