Seprase表达与人结直肠癌临床病理特征的关系  

Relationship Between Expression of Seprase and Clinicopathologic Characteristics in Colorectal Cancer

作　　者：张雪峰[1] 金鑫[2] 吴国强[1] 王希泽[1] 李谨[1] 宗修锟[1] 

机构地区：[1]沈阳军区总医院普通外科,沈阳110016 [2]中国医科大学药理系,沈阳110001

出　　处：《中国普外基础与临床杂志》2006年第1期89-93,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨Seprase表达与结直肠癌临床病理特征间的关系。方法分别采用免疫组织化学、免疫印迹及半定量免疫组织化学方法对50例结直肠癌患者肿瘤标本中Seprase的表达进行了检测,并对Seprase的表达与其临床病理特征的关系进行了分析。结果Seprase在肿瘤细胞和邻近基质细胞中均有表达,Seprase蛋白在肿瘤组织中的表达明显高于正常结直肠组织(P<0.01),其表达与肿瘤Dukes分期及淋巴结转移有关。结论Seprase在结直肠癌中高表达与肿瘤Dukes分期及淋巴结转移有关。Objective To investigate the relationship between expression of Seprase and the clinicopathologic characteristics in colorectal cancer. Methods The expression of Seprase in 50 cases of colorectal cancer was detected with immunohistochemistry, Western-blotting technique and semi quantitative immunohistochemistry, and the relationship between the expression of Seprase and the clinicopathologic characteristics （age. gender, tumor location, tumor size, gross appearance, depth of infiltration, histological classification, lymph node metastasis, venous infiltration, Dukes stage, distant metastasis） was analyzed. Results The expression of Seprase was found both in tumor cells and adjacent stromal cells. The level of Seprase protein was higher in cancer tissue than that in normal tissue, and a semi-quantitative assessment of the immunohistochemistry revealed a significant correlation between Seprase expression and the Dukes stage and the lymph node metastasis. Conclusion The abundant expression of Seprase in colorectal cancer tissue is associated with the Dukes stage and the lymph node metastasis.

关 键 词：SEPRASE 结卣肠癌 转移 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]