杨梅素对淋巴细胞活化及增殖的影响  

The effects of myrictin on inhibition of mouse lymphocytes activated in vitro

作　　者：俞瑜[1] 曾耀英[1] 刘良[2] 季煜华[1] 邢飞跃[1] 肇静娴[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632 [2]香港浸会大学中医药学院

出　　处：《中国药理学通报》2006年第1期63-66,共4页Chinese Pharmacological Bulletin

基　　金：香港特区政府大学教育资助委员会基金资助项目(No2144/03M);广东省自然科学基金资助项目(No04300653)

摘　　要：目的研究杨梅素(myricetin)对小鼠T细胞活化及增殖的影响,探讨其作为免疫调节药物开发的可能性并提供相关实验依据。方法无菌分离小鼠淋巴结细胞,加入不同浓度的杨梅素预先孵育1h,用多克隆刺激剂刀豆蛋白(ConA)刺激T细胞活化,利用荧光标记的单克隆抗体双染技术,流式细胞仪检测杨梅素对小鼠CD3+T细胞CD69表达的影响;采用3H-TdR参入法检测杨梅素对淋巴细胞增殖的影响,采用半定量RT-PCR技术从mRNA水平检测杨梅素对IL-2mRNA表达的影响。采用ELISPOT检测杨梅素对IFN-γ分泌的影响。结果杨梅素能抑制T细胞早期活化标志CD69的表达,并能抑制淋巴细胞增殖反应,同时对淋巴细胞活化后IL-2 mRNA的表达及IFN-γ的分泌也有抑制作用。结论杨梅素对淋巴细胞的活化增殖反应具有抑制作用。Aim To study the effects of myricetin on murine lymphocytes activated. Methods We isolated and cultured the mouse lymphocytes from lymph nodes of mice in vitro. The lymphocytes were pre-treated with myricetin for 1 h prior to activation with ConA. After 6 hour stimulation, IL-2 mRNA expression was determined using semi-quantitative RT-PCR. CD69 expression of mouse CD3^＋ T cell was determined using multicolor flow cytometric analysis. T cells proliferation was detected using ^3H-TdR incorporation and IFN-γ secretion is determined with ELISPOT assay. Results 5 - 100 μmol · L^-1 myricetin inhibited CD69 expression of mouse CD3^＋ T cell, 12. 5 ～ 100 μmol · L^-1 myricetin inhibited proliferation of mouse lymphocytes. 100 μmol · L^-1 myricetin completely inhibited IL-2 mRNA expression of mouse lymphocyte, 50 μmol · L^-1 myricetin and 10 μmol · L^-1 myricetin also inhibited IL-2 mRNA expression. 1 μmol · L^-1 and 10 μmol · L^-1 myricetin also inhibited IFN-γ secretion stimulated by ConA. Conclusion Myricetin may has the inhibitory effects on lymphocyte activation and proliferation.

关 键 词：杨梅素 淋巴细胞活化 增殖 细胞因子 

分 类 号：R-332[医药卫生] R282.71