苯肾上腺素诱导乳鼠心肌细胞蛋白质表达变化的蛋白质组分析  

Proteomic Analysis of Proteins with Altered Expression Induced by Phenylephrine in Neonatal Rat Cardiomyocyte

作　　者：刘宁[1] 李子健[2] 刘志强[1] 刘淑莹[1] 韩启德[2] 张幼怡[2] 

机构地区：[1]中国科学院长春应用化学研究所新药研究实验室长春质谱中心,长春130022 [2]北京大学第三医院血管医学研究所分子心血管学教育部重点实验室,北京100083

出　　处：《高等学校化学学报》2006年第1期79-81,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20273067;30400552);中国科学院知识创新工程重要方向项目(批准号:KGCX2-SW-213-06);国家重点基础研究发展规划项目('九七三'计划)(批准号:G2000056906)资助

摘　　要：In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods,including two dimensional polyacrylamide gel electrophoresis,PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.The good two-dimensional pattern with a high resolution and reproducibility was obtained.Totally 14 differential protein spots were found. Out of these,11 proteins were preliminarily identified.Desmin,Txndc5,etc.were found to be up-regulated in PE-treated cardiomyocytes,while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase,etc.were found to be down-regulated.It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine（PE） , the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods, including two dimensional polyacrylamide gel electrophoresis, PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS. The good two-dimensional pattern with a high resolution and reproducibility was obtained. Totally 14 differential protein spots were found. Out of these, 11 proteins were preliminarily identified. Desmin, Txndc5, etc. were found to be up-regulated in PE-treated cardiomyocytes, while caspase-11,4-hydroxyphenylpyruvic acid dioxygenase, etc. were found to be down-regulated. It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.

关 键 词：苯肾上腺素 心肌细胞 蛋白质组 

分 类 号：O657.63[理学—分析化学] Q51[理学—化学]