冷冻法测定梨的石细胞含量  

Study on the conditions for measuring stone cell content in pear flesh by freezing method

作　　者：聂继云[1] 李静[1] 杨振锋[1] 张红军[1] 李明强[1] 

机构地区：[1]中国农业科学院果树研究所农业部果品及苗木质量监督检验测试中心,辽宁兴城125100

出　　处：《果树学报》2006年第1期133-135,共3页Journal of Fruit Science

基　　金：农业部2004年农业行业标准制定项目(农财发[2004]56号)。

摘　　要：以南果、安梨、砀山酥、秋白、锦丰、五九香、巴哈胜利、丰水、黄金、黄冠等10个梨品种果实为试材,对梨石细胞含量冷冻处理测定法的果肉切块大小、冷冻处理时间、匀浆时间、漂洗方法、平行性与准确度等进行了优化和系统研究,提出最适测定条件为果肉切块大小1.0cm3、-16～20℃冷冻处理24h、22000r/min匀浆3min。在此条件下,石细胞平均回收率为85.1%～94.1%,方法误差为4.65%～11.56%。研究结果为制定科学、实用的梨石细胞含量测定方法农业行业标准奠定了基础。Ten pear cultivars, Nanguo, Anli, Dangshansu, Qiubai, Jinfeng, Wujiuxiang, Packhams, Housui, Whangkeumbae and Huangguan were used for studying the conditions for exactly measuring the stone cells in pear flesh by freezing method. Results showed that the best conditions needed for exactly measuring the stone cell content in pear flesh were as follows： 1.0 cm^3 pulp dollop, frozen under -16℃～20℃ for 24 hours, and blended 22000 r/min for 3 rain; under such conditions the recovery rate of stone cell was 85.1%～94.1%, and the relative deviation of the method was 4.65%～ 11.56%. Such research achievement could be used for the establishment of the scientific and applied National Agriculture Industry Standard for measuring stone cells in pear flesh.

关 键 词：梨 石细胞含量 冷冻法 测定条件 

分 类 号：S661.2[农业科学—果树学]