早期糖尿病大鼠视网膜神经节细胞逆行轴浆流损害的研究  

Damage of the retrograde axial flow of retinal ganglion cells in diabetic rats at the early stage

作　　者：秦要武[1] 徐格致[1] 王文吉[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院,上海200031

出　　处：《中华眼底病杂志》2006年第1期4-6,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的观察早期糖尿病大鼠视网膜神经节细胞(RGC)逆行轴浆流速是否受损,并判断损伤细胞的类型。方法Sprague-Dawley(SD)成年雄性大鼠6只,腹腔注射链脲佐菌霉素(STZ)形成糖尿病模型,4周时行荧光金(FG)上丘定位逆行标记。分别于FG注射后12、72h行视网膜铺片,在荧光显微镜下拍照后做尼氏染色,统计FG标记的不同大小RGC比例。另6只正常SD成年雄性大鼠作为对照。结果FG注射12h后,实验组RGC总数与对照组无差别,但小直径RGC比例明显低于对照组;72h后,实验组RGC数比对照组明显减少,小细胞减少尤其明显。结论糖尿病早期大鼠RGC逆行轴浆流速受到影响,小直径RGC容易受损。Objective To observe whether the retrograde axial flow of retinal ganglion cells （RGC） in diabetic rats at the early stage was damaged. Methods Diabetic model was induced by streptozotocin in 6 adult male Sprague-Dawley （SD） rats. Fluorogold （FG） was injected to the superior eollieuli 4 weeks later. Streched preparation of retina was made 12 and 72 hours after the injection, and was stained after photographed by fluorescent microscope. The proportion of RGC with different sizes labeled by FG was calculated. Other 6 normal adult male SD rats were in the control group. Results Twelve hours after injection with FG, there was no difference of the total number of RGC in experimental and control group, but the ratio of small RGC was lower in experimental group than that in the control group： 72 hours after injection with FG, The number of RGG, especially the small RGC, decreased obviously in experimental group compared with the control group. Conclusion The speed o＇f the retrograde axial flow of RGC in diabetic rats at the early stage is affected, and the small RGC are damageable.

关 键 词：糖尿病/病理生理学 视网膜神经节细胞 染色与标记 

分 类 号：R774.1[医药卫生—眼科]