端粒酶启动子调控p53基因表达对T24细胞的影响  

Effect of expression of p53 gene driven by telomerase gene promoter to bladder carcinoma cell T24

作　　者：朱晓应[1] 符伟军[1] 洪宝发[1] 林茂虎[2] 

机构地区：[1]解放军总医院泌尿外科,北京100853 [2]解放军第304医院泌尿外科

出　　处：《中华实验外科杂志》2006年第2期193-194,共2页Chinese Journal of Experimental Surgery

基　　金：中国博士后科学基金资助项目(2004035285);国家自然科学基金资助项目(30300349)

摘　　要：目的探讨端粒酶催化亚基(hTERT)启动子调控p53基因在膀胱肿瘤细胞T24中表达对细胞的影响。方法将成功构建的phTERT-p53载体,瞬时转染膀胱肿瘤T24细胞;应用流式细胞仪观察细胞凋亡率变化,透射电镜下观察细胞凋亡形态学改变。结果hTERT启动子调控p53基因表达使细胞凋亡率明显升高[24、48h凋亡率实验组15.42%、36.57%;转染绿色荧光蛋白基因(GFP)组5.16%、8.19%;阴性对照组4.49%、7.18%],电镜下观察到典型凋亡改变,凋亡指数达25%。结论hTERT启动子能够激活phTERT-p53载体表达p53,使肿瘤细胞凋亡增加。Objective To explore the effect of expression of p53gene driven by human telomerase reverse transcriptase （hTERT） on human bladder turrnor cell T24. Methods The expression vector of p53 gene afforded by hTERT promoter was constructed and transiently transfected into T24 ceils, and the effect on apoptosis rate of tumor cells was detected by using flow cytometry （FCM） and morphological change under a transmission electron microscope. Results The expression of p53 driven by hTERT promotet increased apoptosis rate （apoptosis rate after 24/48 h：p53：15.42 %/36.57 % ;green fluorescence protein （GFP） ： 5.17%/8.19% ; negative control： 4.49%/7.18% ）. Typical morphological changes of apoptosis were observed under a transmission electronic microscope, and apoptosis index was 25 %. Conclusion The expression of p53 gene under the control of hTERT promoter can increase apoptosis rate of bladder carcinoma cells.

关 键 词：启动子 P53 端粒酶 

分 类 号：R73-3[医药卫生—肿瘤]