PTEN基因治疗鼠C6胶质瘤的体内实验研究  

Treatment of the murine C6 glioblastoma by PTEN:an in vivo study

作　　者：王虎[1] 浦佩玉[2] 康春生[2] 董伦[2] 王广秀[2] 李捷[2] 

机构地区：[1]天津市环湖医院神经外科,300052 [2]天津医科大学总医院神经外科

出　　处：《中华实验外科杂志》2006年第2期204-206,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(39970338)

摘　　要：目的探讨PTEN基因在动物体内对脑胶质瘤的生长作用。方法将大鼠C6胶质瘤细胞(对照组)和转染PTENcDNA的C6细胞(转染组)种植于SD大鼠右侧尾状核,荷载C6脑胶质瘤鼠用PTENcDNA原位治疗(治疗组),并以空载体治疗作为对照(空载组),每组10只,观察大鼠的一般情况、生存期、肿瘤体积变化以及肿瘤病理组织学与细胞生物学特征变化。结果对照组和空载组大鼠均于3周内死亡,而转染组5只大鼠和治疗组6只大鼠观察60d内无自然死亡,生存期较对照组明显延长(P<0.01)。结论PTEN基因在动物体内可以抑制脑胶质瘤的生长,可以成为恶性胶质瘤基因治疗的优选靶之一。Objective To study the of effect of PTEN on growth of C6 glioma cells in vivo. Methods Parental C6 cells and C6 cells trasfected with PTEN were implanted stereotactically into the right eaudate nucleus of SD rats as control and transfeeted groups resepctively. The dynamic MRI and histopathological changes of the tumors and the expression of PTEN in glioma cells were examined. Reo suits In C6 control group and empty vector group, the mean survival was 17.80 ± 0.92 and 17.50 ± 1.10 days respectively, Six rats kept alive and vigorous in 60 days after PTEN lipofeetamine-DNA complex treatment. In transfected group, 5 rats survived up to 60 days. The survival time in PTEN treated group was significantly prolonged （ P 〈 0.01 ）. Conclusion PTEN plays a critical role in the malignant progression of gliomas and may be an important candidate for gene therapy of human gliomas.

关 键 词：PTEN 胶质瘤 基因治疗 

分 类 号：R739.4[医药卫生—肿瘤]