高锌对Caco-2细胞铁、锌含量及其调控基因mRNA表达的影响  

Effect of high zinc concentrations on contents of iron and zinc and expression of their regulating mRNA in Caco-2 cells

作　　者：张弌[1] 李正银[1] Li Jing 秦海宏[1] 

机构地区：[1]第二军医大学海军医学系军队卫生学教研室,上海200433 [2]Department of Chemistry & Biochemistry, University of Texas at Austin

出　　处：《第二军医大学学报》2006年第1期41-45,共5页Academic Journal of Second Military Medical University

基　　金：第二军医大学海军医学系创新研究工程基金.~~

摘　　要：目的:探讨高锌对小肠细胞铁、锌含量和铁、锌调控基因DMT1、IREG1、ZnT1及hZIP4 mRNA表达的影响。方法:以Caco-2细胞为小肠细胞体外模型,以锌浓度分别为4、50、100、200μmol/L的培养液培养24 h,用原子吸收分光光度计检测细胞内铁、锌含量,用RT-PCR方法检测DMT1、IREG1、ZnT1及hZIP4 mRNA表达,以GAPDH mRNA水平作为内参照。结果:高锌处理24 h后Caco-2细胞内锌含量升高,培养基锌浓度为50μmol/L时达到高峰,而细胞内铁含量却随培养基锌浓度升高而降低。细胞内DMT1I、REG1、ZnT1 mRNA表达均出现上调,hZIP4 mRNA表达则发生下调。结论:补锌可以提高小肠细胞内锌含量,但会使细胞内铁含量下降。铁和锌在小肠中的吸收可能相互影响。Objective：To observe the effect of high zinc concentrations on contents of iron and zinc and expression of their regulating mRNA in Caco-2 cells. Methods： Caco- 2 cells were used as model of human small intestinal enterocytes and were treated for 24 h with 4, 50,100 and 200 μmol/L of zinc. The zinc and iron contents were determined by atom absorption spectrophotography and the expression of DMT1, IREG1, ZnT1 and hZIP4 mRNA were determined by RT PCR（the level of GAPDH was taken as internal control）. Results： The zinc content in Caco- 2 cells was increased 24 h after treatment with zinc, and reached the peak when exposed to 50 μmol/L zinc. The iron content decreased with the increase of zinc concentration. Zinc supplement increased the expressions of DMT1, IREC-1 and ZnT1 mRNA hut decreased the expression of hZIP4 mRNA. Conclusion： It is suggested that zinc supplement can increase the contents of zinc but decrease the contents of iron in Caco 2 cells. There may be interactions between iron and zinc in the intestinal lumen.

关 键 词：锌元素 铁元素 CACO-2细胞 DMT1 IREG1 ZnT1 hZIP4 基因表达 

分 类 号：R151.2[医药卫生—营养与食品卫生学]