阿朴吗啡诱导黑质毁损大鼠腹侧被盖区c-jun表达  

Apomorphine induce c-jun expression in ventral tagmental area of 6-OHDA-lesioned rats

作　　者：陈晓宇[1] 姚玉芹[2] 沈韶辉[1] 韩卉[3] 

机构地区：[1]安徽医科大学组织胚胎学教研室 [2]安徽医学高等专科学校 [3]安徽医科大学解剖学教研室,合肥230032

出　　处：《中国临床解剖学杂志》2006年第1期81-83,共3页Chinese Journal of Clinical Anatomy

基　　金：安徽省教育厅自然科学研究基金(No.2003kj187)

摘　　要：目的:观察6-羟基多巴胺(6-hydroxydopamine,6-OHDA)毁损黑质DA能神经元后,不同时间点腹腔注射阿朴吗啡(Apomorphine,APO)大鼠行为学及中脑腹侧被盖区(ventraltagmentalarea,VTA)形态学、c-jun表达情况,探讨其可能机制。方法:6-OHDA单侧一点注射大鼠右黑质致密区(substantianigracompacta,SNc),特异性毁损DA能神经元;术后1、3、7、14、21d腹腔注射APO,观察旋转行为;利用电镜、尼氏染色、免疫组织化学ABC法,观察各时间点VTADA能神经元形态学变化和酪氨酸羟化酶(TH)、c-jun表达情况。结果:毁损侧VTADA能神经元逐渐减少,超微结构损伤逐渐加重;DA神经元丢失≥75%时,APO诱导的旋转实验≥7r/min,VTA毁损侧c-jun表达。结论:APO能诱导毁损侧VTA表达c-jun;c-jun表达与DA能神经元毁损程度有一定的关系。Objective ： To observe the c-jun expression in ventral tagmental area （VTA） of 6-hydroxyd- opamine （6-OHDA）-lesioned rats after apomorphine （APO） intraperitoneal injection（ip） and to investigate the inherent mechanism of them. Methods： Rats received a unilateral injection of 6-OHDA into right substantia nigra compacta （SNc）. The abnormal rotations induced by APO were investigated at 1, 3, 7, 14 and 21d after lesion. Dopaminergic derogation and c-jun expression were detected by electronmicroscope,Nissl＇s body staining, and immunohistochemical avidin-biotinylated peroxidase complex method （ABC） to explore the changes of tyrosine hydroxylase （TH）and the expression of c-jun in DA neurons. Results： The number of dopaminergic neurons decreased gradually, and its ultmmicrostructure was severely damaged by degrees in the lesioned site. Dopaminergic neurons lossed over 75%, contralateral rotations surpassed 7r/min, while the expression of c-jun could be tested in VTA. Conclusions： APO can induce c-jun expression in VTA. Intrinsic relations may be existed between dopaminergic neurons deletion and c-jun expression.

关 键 词：6-羟基多巴胺 阿朴吗啡 中脑腹侧被盖区 酪氨酸羟化酶 c-jun 

分 类 号：R749.16[医药卫生—神经病学与精神病学]