皮肤恶性黑素瘤中基质金属蛋白酶-9及其组织抑制物-1的表达及意义  

Expression and significance of matrix metalloproteinase-9 and its tissue inhibitor in cutaneous malignant melanoma

作　　者：李新功[1] 孙莉[1] 刘永[1] 徐志秀[1] 常峰[1] 吴起嵩[1] 董艳光[1] 李爱英[1] 

机构地区：[1]胜利油田中心医院

出　　处：《中国麻风皮肤病杂志》2006年第1期8-10,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:探讨基质金属蛋白酶-9(MMP-9)及其组织抑制物-1(TIMP-1)在皮肤恶性黑素瘤中的表达及其临床、病理学意义。方法:采用免疫组织化学S-P方法观察MMP-9和TIMP-1在51例皮肤黑素瘤及15例色素痣石蜡切片中的表达,使用图像分析技术对免疫组化结果进行定量分析。结果:与色素痣比较,皮肤恶性黑素瘤中MMP-9表达较强(P<0.05),MMP-9/TIMP-1比值较高(P<0.05)。MMP-9和TIMP-1的表达及MMP-9/TIMP-1比值与黑素瘤浸润深度有关(P<0.05)。有淋巴结转移者MMP-9表达较强(P<0.05),MMP-9/TIMP-1比值较高(P<0.05)。结论:MMP-9、TIMP-1蛋白的表达与皮肤恶性黑素瘤的侵袭性有关,MMP-9/TIMP-1比值增高提示肿瘤具有较高侵袭转移能力。Objective： To investigate the expression of matrix metalloproteinase - 9 （MMP- 9） and its tissue inhibitor （TIMP- 1 ） in cutaneous malignant melanoma and its clinicopathological significance. Methods： Immunohistocbemieai method of streptavidin - peroxidase was used to observe the expression of MMP - 9 and TIMP - 1 proteius on the paraffin embedded sections in 51 cases with cutaneous melanoma and 15 cases with nevus, The immunohistoehemieal results were analyzed quantitatively using an image analysis system. Results： The expression of MMP - 9 and the MMP-9/TIMP- 1 ratio was higher in cutaneous malignant melanoma than that in nevus （P 〈 0.05）. The expression of MMP - 9 and TIMP - 1 and the MMP - 9/TIMP - 1 ratio were close related （ P 〈 0.01, P 〈 0.05 and P 〈 0.05） to the depth of invasion of cutaneous malignant melanoma. The expression of MMP- 9 was stronger and the MMP- 9/ TIMP- 1 ratio was higher in the patients with positive lymph node metastasis than that in the negative group （ P 〈 0.05）. Conclusion： The expression levels of MMP- 9 and TIMP- 1 protein correlates with the aggressive behavior of cutaneous malignant melanoma, and the higer ratio of MMP - 9/timp - 1 may indicate a higer risk of the tumor invasion and metastasis.

关 键 词：皮肤恶性黑素瘤 明胶酶B 金属蛋白酶类组织抑制剂 

分 类 号：R739.5[医药卫生—肿瘤]