补充活性肽对大鼠1次离心运动后骨骼肌微细损伤作用的形态学研究  

Effects of Soy Hydrolyzed Peptides Supplementation on Histological and Ultrastructural Damages of Skeletal Muscle after a Bout of Eccentric Exercise in Rats

作　　者：李世成[1] 李跃纲[2] 王启荣[3] 杨则宜[3] 李肃反[3] 

机构地区：[1]广东省湛江师范学院体育科学学院,广东湛江524048 [2]北京市疾病控制中心 [3]国家体育总局运动医学研究所

出　　处：《中国运动医学杂志》2006年第1期31-36,共6页Chinese Journal of Sports Medicine

基　　金：国家科技攻关计划奥运科技专项(2002BA904B16)资助

摘　　要：目的:本研究从形态学角度,观察补充活性肽对大鼠运动后骨骼肌微细损伤的作用。方法:雄性SD大鼠150只,按照补充不同物质(水、大豆分离蛋白和活性肽),运动与不运动,以及运动后0 hr、12 hr2、4 hr和48hr不同时间随机分为15组,所有动物平衡膳食1周后进行实验。运动组大鼠进行持续跑台训练120分钟。实验组和对照组所有动物在平衡膳食期间每天分别以15%活性肽饮料2毫升、15%大豆分离蛋白2毫升和等量的纯净水灌胃。结果:补充活性肽和大豆分离蛋白均可减轻大鼠1次离心运动后骨骼肌微细损伤的形态学改变和超微结构变化,其中活性肽的作用有强于大豆分离蛋白的趋势。Objective Through morphometric methods, the effects of soy hydrolyzed peptides （SHPs） supplementation on histological and ultrastructural damages of skeletal muscle after intensive training were investigated. Methods 150 male Sprague - Dawley rats were randomly divided into 15 groups according to different supplementations （placebo, ISP and SHPs）, exercise or not and different sampling phase after exercise. Rats in exercise groups ran 120 minutes at speed of 20 ± lm/min and - 16° of slope. All rats were respectively fed with 2ml beverage containing 15% SHPs, 15% ISP or placebo for 1 week before exercise. Results HE staining results showed that the skeletal muscle structure changed obviously in 24 - 48hr after exercise and the ultrastructure of skeletal muscle changed evidently in 12 - 24hr after exercise. With ISP or SHPs supplementation, the histological and ultrastructural damages of skeletal muscle reduced obviously. Moreover, SHPs supplementation showed better protective effects than ISP supplementation.

关 键 词：活性肽 骨骼肌 形态学 离心运动 大鼠 骨骼肌微细损伤 

分 类 号：R87[医药卫生—运动医学]