我国部分地区猪源大肠杆菌LEE和HPI毒力岛相关基因的检测  

Detection of related genes of LEE and HPI pathogenicity island of Escherichia coli strains isolated from pigs in China

作　　者：陈祥[1] 赵娟[1] 高崧[1] 苗晓青[1] 刘静[1] 黄莉莉[1] 焦新安[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《中国人兽共患病学报》2006年第1期33-35,47,共4页Chinese Journal of Zoonoses

基　　金：教育部科学技术研究重点项目(No.204050);国家"863"计划(No.2003AA222141)

摘　　要：目的采用PCR方法检测我国江苏等9个省市部分地区分离的1 007株猪源大肠杆菌中肠细胞脱落位点毒力岛(LEE)和强毒力岛(HPI)。方法对LEE毒力岛检测其核心区的ler和eaeA基因,对HPI毒力岛检测其irp2和fyuA基因。同时对LEE和/或HPI阳性大肠杆菌进行了O血清型的鉴定。结果在分离的猪源大肠杆菌中,LEE毒力岛基因检测结果为:2.2%(22/1 007)的菌株ler和eaeA基因的扩增阳性,0.5%(5/100 7)的菌株eaeA阳性。HPI毒力岛基因检测结果为:10.6%(107/1 007)的菌株irp2和fruA基因扩增阳性,2.1%(21/1007)的菌株irp2阳性;其中有2株irp2和ler基因扩增阳性,有3株irp2、ler和eaeA基因扩增阳性,1株irp2f、yuA、ler和eaeA基因扩增都为阳性;在128个HPI阳性分离株中,O93和O107血清型菌株分别占定型菌株的15.7%(13/83)和12.0%(10/83)。结论2.7%的猪源大肠杆菌携带LEE,12.7%的菌株携带耶尔森菌HPI,O93和O107为猪源HPI大肠杆菌常见血清型。This study was undertaken to determine the present distribution of locus of enterocyte effacement pathogenicity island（LEE） and high pathogenicity island（HPI） among strains of E. coli isolated from pigs from 9 provinces in China. One thousand and seven E. coil isolates were collected for the detection of genes of irp2, fyuA, let and eaeA by a multiplex PCR, and serogrouped using O- antisera of E. coli. Among these E. coli isolates, 2.2 % （22/1 007） of the isolates possessed the genes of/er and eaeA, 0.5 % （5/1 007） strains only harboured the gene of eaeA, 10.6% （107/1 007） of strains had the genes of irp2/fyuA and 2.1% （21/1 007） only held irp2o Of all these isolates, two strains possessed the genes of irp2 and ler, three strains harboured the gene of irp2, /er and eaeA, and one strain had the genes of irp2, fyuA, ler and eaeA. The 27 isolates carrying the genes of LEE, these isolates distributed in 11 O serogroups. The 128 isolates containing the genes of HPI distributed in 32 serogroups different , whereas 18.0% of them belonged to 093 and O107. However uncommon O serogroups were also discovered in this study.

关 键 词：猪源大肠杆菌 O血清型 强毒力岛 肠细胞脱落位点毒力岛 

分 类 号：S378[农业科学—农产品加工]