13-甲基肉豆蔻酸抗小鼠宫颈癌U14  

Anticancer Effects of 13-Methyltetradecanoic Acid on Murine Cervical Tumor U14

作　　者：翁绳美[1] 孙建成[1] 黄自强[1] 

机构地区：[1]福建医科大学药学院药理学系,福州350004

出　　处：《福建医科大学学报》2006年第1期33-34,43,共3页Journal of Fujian Medical University

基　　金：福建省青年科技人才创新基金资助项目(2002J045)

摘　　要：目的探讨末端支链脂肪酸13-甲基肉豆蔻酸(13-MTD)抗小鼠肿瘤作用及其对肉毒碱棕榈酰转移酶(CPT-1)活性影响的相关性。方法荷U14肿瘤的ICR小鼠分5组:阴性对照组给水,阳性对照组以环磷酰胺灌胃,13-MTD组分为高、中、低剂量组,每日灌胃,共12 d,取瘤称质量,计算抑瘤率。从各给药组瘤组织提取线粒体,测定各个肿瘤线粒体的CPT-1活性,计算相应肿瘤质量与CPT-1酶活性的相关系数。试管内测定13-MTD对提取线粒体的CPT-1的直接作用。结果13-MTD对U14肿瘤的抑制作用表现为剂量依赖性,高剂量抑制率达64.9%。瘤质量与CPT-1酶活性的相关系数r=0.584,P<0.05。在试管内13-MTD对U14肿瘤的CPT-1酶活性呈剂量依赖的直接抑制作用。结论13-MTD能直接抑制U14肿瘤CPT-1酶,抗肿瘤作用与抑制肿瘤线粒体CPT-1酶活性正相关。Objective To investigate anticancer effects of a branched-chain fatty acid, 13-methyltetradecanoic acid（13-MTD）, and the relationship between the anticancer effects and its inhibition of mitochondria carnitine palmitoyltransferase 1（CPT-1） activity. Methods ICR mice bearing cervical tumor U14 were treated with varied dosage of 13-MTD for 12 d, at the end of experiment, the mice were killed and the tumors were weighted. CPT-1 activity was measured with the mitochondria isolated from the 13-MTD treated tumors, and the correlation between the CPT-1 activities and the related tumor weight was determined. Results 13-MTD significantly inhibited the growth of tumor U14, with a maximum inhibition rate of 64.9%. The correlation between the CPT-1 activity and tumor weight index was r= 0. 584 （ n = 20, P 〈 0.05）. Conclusion 13- MTD can inhibit the growth of murine cervical tumor U14 and inhibit the tumor mitochondfia CPT-1 activity in vitro. Anticancer effect of 13-MTD ascribed to the inhibition of CPT-1 activity.

关 键 词：宫颈肿瘤 肉豆蔻酸 肉毒碱棕榈酰转移酶 小鼠 

分 类 号：R737.33[医药卫生—肿瘤]