聚乳酸聚乙二醇复合骨形成蛋白修复下颌骨缺损  

A study of PLA-PEG/rhBMP-2 block compound used for the repair of mandibular defect

作　　者：王金涛[1] 孙宏晨[2] 梁奇志[3] 陈学思[3] 车延海[2] 

机构地区：[1]河北大学附属医院口腔科,071000 [2]吉林大学口腔医学院 [3]中国科学院长春应用化学研究所

出　　处：《实用口腔医学杂志》2006年第1期17-20,共4页Journal of Practical Stomatology

摘　　要：目的:探讨聚乳酸聚乙二醇嵌段共聚物作为人工骨支架材料的可行性。方法:制备聚乳酸聚乙二醇嵌段共聚物/重组人骨形成蛋白2多孔复合材料,测定其压缩强度和压缩模量;人为制造兔下颌骨缺损模型,分别植入该材料(实验组)和不含骨形成蛋白的聚乳酸聚乙二醇多孔材料(对照组),于2、4、8、16周处死动物,通过组织病理学观察和光密度分析来评价该材料的成骨效果以及制备方法是否合理。结果:复合材料的压缩强度为(30.05±3.12)MPa,压缩模量为(371.67±12.37)MPa;实验组在各个时期均可见到新生骨形成,且新生骨逐渐向材料内部生长,对照组仅在初期有少量新生骨出现;实验组各个时段的光密度明显高于对照组,在16周时实验组缺损区同周围骨组织密度近似(P>0.5)。结论:多孔材料的制备方法未破坏骨形成蛋白活性,复合材料具有较好的体内成骨能力,聚乳酸聚乙二醇嵌段共聚物可作为人工骨支架材料。Objective： To evaluate the properties of PLA-PEG as a biodegradable scaffold material combined with rhBMP-2 for bone construction. Methods：PLA-PEG/rhBMP-2 multipore compound was prepared, then compression intensity and compression module were tested. The mandibular defect model was made in 20 rabbits, then PLAPEG/rhBMP-2 was implanted into the defects on one side, and PLA-PEG without rhBMP-2 was implanted into the defect on another side as the control. The bone specimens were retrieved to make density analysis with X-ray photogram following sacrifice of the rabbits 2, 4, 8 and 16 weeks respectively after operation, then the specimens were decalcified to make histopathological observation by means of HE stain. Results：Compression intensity （MPa） of the PLA-PEG/rhBMP-2 compound was 30.05 ± 3.12, and compression module（MPa） was 371.67 ± 12.37. At 2,4,8 and 16 weeks ,more new bone was observed, and higher intensity（ P 〈 0. 001 ） was observed in PLA-PEG/rhBMP-2 compound transplantations than in PLA-PEG examined by histopathological observation and X-ray analysis. Conclusion：The PLA-PEG/rhBMP block is feasible for the repair of bone defect.

关 键 词：乳酸聚乙二醇嵌段共聚物 重组人骨形成蛋白2 下颌骨缺损 压缩强度 

分 类 号：R782[医药卫生—口腔医学]