脊髓神经干细胞培养及体外诱导分化实验研究  

Experimental study on cultivation and inducement differentiation of spinal cord-derived neural stem cells in vitro

作　　者：褚尤彪[1] 罗卓荆[1] 曹延林[1] 宫伟[1] 龚凯[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2006年第1期19-22,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家863课题基金资助项目(2002AA216101)

摘　　要：目的探讨胚胎大鼠脊髓神经干细胞(SNSCs)的分离培养及体外诱导分化的特点和规律。方法利用无血清培养技术和细胞免疫化学方法。结果在表皮生长因子(EGF),碱性成纤维细胞生长因子(bFGF)的作用下,SNSCs快速增殖,形成多细胞组成的细胞球,将这些细胞球分离成单细胞重新培养,单个的细胞又很快变成细胞球。虽然白血病抑制因子(LIF)不是SNSCs培养所必需的,但是它可明显促进SNSCs增殖。诱导分化实验显示:SNSCs可分化为神经元、星型胶质细胞和少突胶质细胞,而LIF组则分化很少。结论从胚鼠脊髓分离神经干细胞是可行的,这些细胞在体外经多次传代后仍具有较强的增殖能力和多向分化潜能;LIF可以促进SNSCs增殖并抑制其分化。Objective To investigate the characteristics and rules of cultivation and in vitro inducement differentiation of spinal cord-derived neural stem cells （SNSCs） in vitro from rat＇s embryo, Mehods The technique of serum-free cultivation and the methods of immunofluoreseence cytochemistry were used. Results In the presence of epidermal growth factor （EGF） and basic fibroblast growth factor （bFGF）, SNSCs were observed to proliferate rapidly and form some cellular spheres. After separated into single cells, which were then plated, the cellular spheres were observed to be formed again. Although leukemia inhibitory factor （LIF） was not necessary for SNSCs cultivation, it enhanced the proliferation of SNSCs obviously. The experiment of differentiation showed that SNSCs could differentiate into neurons, astrocytes and oligodendrocytes, whereas few SNSCs differentiated with LIF. Conclusion It is feasible to isolate SNSCs of rat＇s embryo. Those cells can expand and proliferate in vitro through multiple passages. LIF can promote the proliferation of SNSCs and inhibit the differentiation of SNSCs in vitro.

关 键 词：神经干细胞 脊髓 白血病抑制因子 分化 

分 类 号：R394.26[医药卫生—医学遗传学]