不同期别子宫内膜癌组织中差异表达基因的层次聚类分析  

Study of differential gene expression in different stage human endometrial cancer

作　　者：万小平[1] 蔡斌[1] 刘玲[1] 席晓薇[1] 杨懿霞[1] 

机构地区：[1]上海交通大学附属第一人民医院妇产科,200080

出　　处：《中华妇产科杂志》2006年第1期38-42,共5页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(30371481)

摘　　要：目的探讨对不同期别子宫内膜癌组织中的差异表达基因进行层次聚类分析的意义。方法利用基因芯片技术分析32例不同期别子宫内膜癌组织中的差异表达基因,并对其基因表达谱进行层次聚类分析。结果根据国际妇产科联盟(FIGO)手术病理分期的不同对所有样本进行分组,分为Ⅰ、Ⅱ、Ⅲ、Ⅳ期组4组,筛选出与肿瘤转移相关的差异表达基因12个。根据这12个差异表达基因对32例内膜癌进行层次聚类分析,其结果与手术病理分期的符合率为66%。结论基因芯片技术可以发现与子宫内膜癌转移相关的差异表达基因,基因表达谱层次聚类分析可以在术前帮助判断高危型子宫内膜癌。Objective To study the gene expression profile of different stage endometrial cancer and identify genes related to the progression and metastasis of endometrial cancer. Methods cDNA microarray was used to analyze the differentially expressed genes between different stage endometrial cancers. The hierarchical cluster analysis was applied to the gene expression profile of 32 endometrial cancers. Results Twelve differentially expressed genes were identified between different stage endometrial cancers. The conformity rate was 66% between the result of hierarchical cluster analysis and the operative-histological stages of Intemation Federation of Gynecology and Obstetrics （FIGO）. Conclusion Genes related to the endometrial cancer progression and metastasis can be identified by differential gene expression profile with cDNA microarray and high-risk endometrial cancer may be distinguished before surgery by hierarchical cluster analysis.

关 键 词：寡核苷酸序列分析 子宫内膜肿瘤 基因表达谱 聚类分析 

分 类 号：R737.33[医药卫生—肿瘤]