PP333与BA组合对怀地黄试管苗生长发育的影响  

The Influence of PP333 in Combination with Benzylaminopurine on Growth of Rehmannia glutinosa Plantlets

作　　者：李明军[1] 徐鑫[1] 夏民[2] 张晓丽[1] 柳俊[3] 谢从华[3] 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]南阳师范学院生物系,南阳473000 [3]华中农业大学园艺植物生物学教育部重点实验室,武汉430070

出　　处：《植物学通报》2006年第1期56-59,共4页Chinese Bulletin of Botany

基　　金：河南省重点科技攻关项目(0423032100)

摘　　要：本文研究了PP333与BA组合对怀地黄(Rehmanniaglutinosa)试管苗生长发育的影响。结果表明:(1)与对照相比,不同浓度的PP333与0.5mg.L-1BA组合均严重地抑制试管苗根、茎和叶的生长,表现为植株矮小,生根晚,前期根畸形生长(呈棒状或球状);(2)与单独使用0.5mg.L-1BA相比,低浓度PP333(0.01和0.1mg.L-1)与0.5mg.L-1BA组合促进茎的伸长和加粗;(3)2mg.L-1PP333与0.5mg.L-1BA组合能显著地提高愈伤组织芽的分化率和腋芽的萌发率,有利于试管苗的快速繁殖。The influence of PP333 with benzylaminopurine （BA） on growth of Rehmannia glutinosa plantlets was studied in this paper. Combinations of （0.5 mg·L^-1） BA and different concentrations of PP333 inhibited the growth of roots, stems and leaves significantly. Plantlets were short and small, and roots appeared later. The roots looked like clubs or balls initially. Compared with （0.5 mg·L^-1） BA, （0.5 mg·L^-1） BA with lower concentrations of PP333, at 0.01 or 0.1 mg·L^-1, improved the extension and thickness of stems. MS medium supplied with （2 mg·L^-1） PP333 and （0.5 mg·L^-1） BA increased the rate of bud formation of callus and pullulation of axillary buds observably, which is good for largescale in vitro propagation.

关 键 词：怀地黄 试管苗 PP333 BA 生长发育 繁殖 

分 类 号：S567.239[农业科学—中草药栽培]