白桦酯醇对食管癌细胞EC109的抑制作用  

Inhibition Effect of Betulin on Oesophageal Cancer EC109 Cell Line

作　　者：蔡唯佳[1] 马云青[2] 祁逸梅[3] 牛永东[1] 许丽艳[1] 李建新[1] 李恩民[1] 

机构地区：[1]汕头大学医学院,广东汕头505130 [2]吉林市实验中学,吉林吉林132011 [3]东北师范大学化学学院,吉林长春130024

出　　处：《癌变．畸变．突变》2006年第1期16-18,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金面上项目(No.30370641);广东省自然科学基金重点项目(No.37788;No.05104541);广东省自然科学基金面上项目(010431);广东省自然科学基金博士科学研究启动基金项目(5301047)

摘　　要：背景与目的白桦酯醇是一种重要的三萜类生物活性分子,本文从白桦树皮中提取获得白桦酯醇纯品,然后研究该生物活性分子抑制食管癌细胞的活性。材料与方法取一定量的白桦树皮干粉,通过乙醇回流,减压浓缩和甲醇-氯仿重结晶等步骤获得白桦酯醇纯品。用不同剂量的白桦酯醇作用于EC109食管癌细胞48h,噻唑蓝(Methylthiazoletetrazolium,MTT)法检测细胞抑制率。结果白桦酯醇作用于食管癌细胞EC109后,EC109细胞形态发生明显改变,细胞抑制率随白桦酯醇浓度的增高而增高,呈现剂量依赖关系。结论白桦酯醇对食管癌细胞EC109有明显抑制活性。BACKGROUND ＆ AIM： Betulin is an important triterpene. We investigated the antitumor activity of betulin, extracted from the bark of silver birch （Betula platyphylla Suk）. MATERIAL AND METHODS： Betulin was isolated from dry powder of the bark of silver birch （Betula platyphylla Suk） by using ethanol circumfluence, decompress concentration and methanol-chloroform recrystallization. The inhibition rate on EC109 cell growth was detected by MTT （Methyhhiazoletetrazolium） method, after the oesophageal cancer EC109 cells have been treated for 48h with different doses of betulin. RESULTS： With the increasing doses of betulin, the inhibition rate of EC109 cell growth was increased, and their morphological characteristics were changed significamtly. The inhibition rate showed dose-dependent relation. CONCLUSION： Betulin had potent inhibiting effects on EC109 cells growth in vitro.

关 键 词：白桦酯醇 食管癌细胞 抗肿瘤活性 

分 类 号：R735.1[医药卫生—肿瘤]