利用基因芯片技术对大鼠角膜新生血管基因表达谱的研究  

A study on gene expression profile of corneal neovascularization in rat with microarrays

作　　者：赵军[1] 黄一飞[1] 陈学国[1] 李朝辉[1] 何守志[1] 

机构地区：[1]解放军总医院眼科,北京100853

出　　处：《眼科研究》2006年第1期43-45,共3页Chinese Ophthalmic Research

摘　　要：目的运用基因芯片技术研究大鼠角膜新生血管的基因表达谱。方法40只SD大鼠在实验眼角膜缝线后第4d和第12d处死。用有5705条基因的Oligo芯片检测角膜组织的基因表达谱。结果缝线后第4d,5705条基因中,差异表达基因为57条,其中上调29条,下调28条(ratio>4或<0.25)。上调明显的基因有SCYA2、S100A9、MMP3、TIMP1、IL1R2等;下调明显的基因有GALE、GATM和GALD1等。缝线后第12d,差异表达基因为38条,其中上调34条,下调4条。上调明显的基因有MMP3、MMP12、MMP13、TIMP1、SCYA2、S100A9等。下调明显的基因有ALDH1A1等。第4d和第12d上调的基因明显多于下调的基因。结论角膜新生血管的形成是许多不同因子共同调控的结果。Objective To identify gene expression pattern of corneal neovascularization in rat with microarrays. Methods Corneal angiogenesis was provoked by cornea suture. Forty SD rats were subdivided into 4-day group and 12-day group according to their sacrificing time. The genetic expression profile of cornea tissue was detected with Oligo microarrays containing 5 705 genes. Results Fifty-seven of 5 705 genes had differentiation expression on day 4, and 29 of them were upregulated genes and 28 were downregulated genes（ ratio 〉4 or 〈 0.25 ）. The majority of upregulated genes was SCYA2,S100A9, MMP-3, TIMP-1 and IL1R2 and that of downregulated genes was GALE, GATM and GALD1. There were 38 differentiation expression genes among the 5 705 genes more than fourfold on day 12, and 34 of them were upregulated and 4 were downregulated genes. The majority of upregulated genes was MMP-3 ,MMP-12 ,MMP-13 ,TIMP-1 ,SCYA2 and SIOOA9 and that of downregulated genes was ALDH1A1. The upregulated genes were significantly more than the downregulated ones. Conclusion The formation of corneal neovascularization is a result of common regulation by many different factors.

关 键 词：基因芯片 角膜新生血管 差异表达基因 大鼠 

分 类 号：R772[医药卫生—眼科]