牛磺酸治疗糖尿病大鼠骨骼肌病变的实验研究  

The experimental study of Taurine treatment on the affected skeletal muscle in diabetic rats

作　　者：杨丽娟[1] 刘磊[2] 祈真玉[1] 

机构地区：[1]蚌埠医学院生理学教研室,安徽蚌埠233003 [2]蚌埠医学院附属医院肾病科

出　　处：《解剖与临床》2006年第1期31-32,37,共3页Anatomy and Clinics

基　　金：安徽省教育厅自然科学研究资助项目(2005kj279)

摘　　要：目的:研究牛磺酸对糖尿病大鼠骨骼肌酶的影响。方法:SD大鼠24只,随机分为对照(NC)组、糖尿病(DM)组、牛磺酸(Tau)组,每组8只。DM组和Tau组腹腔注射链脲菌素(STZ)50mg/kg复制DM大鼠模型。NC组和DM组给予自来水、Tau组给予牛磺酸(1%饮水)4周后,测量腓肠肌组织中的乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、肌酸激酶(CK)的活性及丙二醛(MDA)含量的变化;光镜下观察肌纤维病理改变。结果:与NC组相比,DM组组织中SOD、CK活性显著降低(P<0.01),LDH活性、MDA含量显著增加(P<0.01);光镜下,肌纤维发生明显紊乱,变细,甚至断裂。给予牛磺酸后可抑制上述现象。结论:牛磺酸对糖尿病大鼠骨骼肌病变有保护作用。Objective ：To investigate the effect of Taurine on some enzyme of skeletal muscle in diabetic rats. Methods：Twenty -four SD rats were randomly divided into control（NC） group, diabetes mellitus（DM） group and taurine treatment（Tau） group. Rats from DM group and Tau group were given 50mg/kg streptozotocin（STZ） by intraperitoneal injection. Ths rats in NC and DM group had been intaking tap water, while the rats from Tau group sipping 1% taurine in aqueous sulution for 4 weeks. The activitiy of lactate dehydrogenase（LDH）, superoxide dismutase（SOD） and creatine kinase（CK）, and the contents of malonaldehyde（MDA） in gastrocnemius muscle tissues were measured. The pathologyical change of muscle fibers were observed. Results：The activity of SOD and CK in the tissues was obviously reduced （P 〈 0,01 ）, LDH activities and MDA contents were obviously increased（ P 〈 0.01 ） in tissue from DM group compared with NC group. Under light microscope, muscle fiber present as disorder, thinning even breakage. Above-mentioned phenomenon could be depressed by taking Taurine. Conclusion：Taurin can protect the injury of skeletal muscle in diabetic rats.

关 键 词：牛磺酸 糖尿病 大鼠 腓肠肌 

分 类 号：R587.1[医药卫生—内分泌]