血清胸腺因子对放化疗保护作用的实验研究  

Experimental study on radioprotective and cytoprotective activities of facteur thymic serique in mice

作　　者：李湛军[1] 廖晓全[1] 徐康森[1] 

机构地区：[1]中国药品生物制品检定所生化药品与基因工程药物室,北京100050

出　　处：《中国临床药理学与治疗学》2006年第1期70-73,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察血清胸腺因子在对抗辐射和抗化疗副作用方面的作用。方法:抗辐射实验:KM小鼠,共6组,除空白组外,其它小鼠接受钴(Co)60照射后,模型组皮下(S.C.)注射0.1 ml生理盐水,FTS低、中、高剂量组分别S.C.注射FTS 0.025、0.25、2.5mg.kg-1,每天一次,连续7 d;阳性药组于实验d 1一次性口服灌胃尼尔雌醇2.5 mg.kg-1,检测免疫活性;对分别接受Co60的600或700拉德辐射小鼠,记录30 d内存活率。抗化疗副作用实验:对肝癌H22细胞的荷瘤小鼠,当日腹腔注射环磷酰胺100mg.kg-1,同时各组给药,每天一次,连续19 d,记录20 d内小鼠存活率。结果:小鼠经辐射后,与模型组比较,FTS各剂量组白细胞总数、对碳粒的吞噬速率和吞噬指数、胸腺系数显著提高(P<0.05);脾脏系数比尼尔雌醇组的脾脏系数略高(P>0.05);对700拉德的辐射小鼠,与模型组比,FTS 0.25 mg.kg-1组30 d内存活率显著增加(P<0.05)、平均存活天数明显延长(P<0.01),与阳性药组一致。荷瘤小鼠接受环磷酰胺化疗,皮下注射FTS给予荷瘤小鼠经化疗后的20 d内存活率增加。结论:血清胸腺因子具有抗辐射、抗化疗副作用,前景广阔。AIM： To evaluate the radioprotective effects and cytoprotective activities of the facteur thymic serique （FTS） to reduce certain side-effects in the chemotherapy in mice. METHODS： Except of control group, all mice which received acute whole-body Co-60-gamma irradiation were randomized to 5 groups： Saline solution was given to model group, 0.025, 0.25, and 2.5 mg· kg^- 1·day^- 1 for 7 consecutive days FTS to low or medial or large dose of FTS group by s. c. , 2.5 mg·kg^-1 nilestriol to control drug group by i.g. at 1 st day and the radioprotective efficacy of FTS was determined by immunity effects and by measuring 30-day survival at 6.0 Gy or 7.0 Gy. The same doses above of FTS were investigated by measuring 20-day survival at 100 mg·kg^-1d^-1 for 14 consecutive days cyclophosphamide injury in mice with H22 liver cancer cell for its cytoprotective activities to reduce certain side-effects in the chemotherapy of cancer. RESULTS： The leucocyte total number, phagocyte competence and thymus index were enhanced significantly when compared with the model group （ P 〈 0.05）, but spleen index increased than those of nilestriol group （ P 〉 0.05）. Thirty-day survival for 0.25 mg·kg^-1 FST administered mice at 7.0 Gy was significantly increased in comparison with saline administered mice （P 〈 0.05） and the survival days were longer （P 〈0.01）, the same as nilestriol group. 20-day survival was increased for FST to inhibit cyclophosphamide-induced injury in mice with tumors. CONCLUSIONS： FTS developed in our laboratory provides significant protection from acute whole-body gamma irradiation or chemotherapy （cyclophosphamide） injury in mice. It may play an important role in the manifestation of its radioprotective and cytoprotective efficacy.

关 键 词：血清胸腺因子 抗辐射 免疫系统 荷瘤小鼠 化疗 存活率 

分 类 号：R965.1[医药卫生—药理学]