端粒DNA形成鸟嘌呤四链体结构  

Experimental Study of Guanosine-Quardruplex Formation from Telomeric DNA

作　　者：胡晓文[1] 黄洪章[2] 傅玉茹[3] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院,广东广州510055 [2]中山大学附属第二医院口腔颌面外科,广东广州510120 [3]中山大学附属第二医院研究中心,广东广州510120

出　　处：《中山大学学报（医学科学版）》2006年第1期15-18,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：中国博士后基金资助项目(2003033421)

摘　　要：目的在体外观察K+、Na+对富含端粒重复序列寡核苷酸二级结构的影响。方法在K+、Na+存在下,利用核磁共振光谱仪观察含端粒重复序列的核苷酸d(TTAGGGT)光谱的变化;利用非变性凝胶电泳观察d(TTAGGG)3、d(TTAGGG)4和d(TTAGGG)5迁移速度的变化。结果在含K+或Na+缓冲液中,d(TTAGGGT)在化学移位δ11～12区域内出现代表有鸟嘌呤四分体形成的波峰;K+比Na+具有更强的稳定四链体能力。K+、Na+均可加速d(TTAGGG)4、d(TTAGGG)5在凝胶中迁移速度。结论在体外,K+、Na+可诱导含端粒重复序列的核苷酸形成鸟嘌呤四链体结构。[Objective] To observe the effects of K^＋ or Na^＋ on the second structure of the ollgonucleotides containing telomeric repeats in vitro. [Methods] In the presence of K^＋ or Na^＋ , the spectrum changes of the oligonucleotide d （TTAGGGT） containing telomeric repeats were observed using nuclear magnetic resonance （NMR） spectrometer. The mobility of d（TTAGGG）3, d（TTAGGG）4 and d（TTAGGG）5 were surveyed using polyacrylamide gel electrophoresis under non-denaturing condition. [ Results] In the buffers consisting of K^＋ or Na^＋ , the nucleotide d （TTAGGGT） showed three peaks meaning G-quartet formation within the ＂chemical shift＂ field of 11-12 ppm ; and the ability that K^＋ stabled G-quadruplex structure was more powerful than that of Na^＋. Both K^＋ and Na^＋ were able to speed mobility of d（TTAGGG）4 and d（TTAGGG）5 in the gel eletrophoresis. [Conclusion] Both K^＋ and Na^＋ are able to induce the oligonucleotides containing telomeric repeats to form G-quadruplex structure in vitro.

关 键 词：端粒 鸟嘌呤四链体 钾离子 钠离子 

分 类 号：R782.1[医药卫生—口腔医学]