抗α_1-肾上腺素能受体抗体对培养大鼠主动脉平滑肌细胞增殖的影响  

Effects of the antibodies against α_1-adrenergic receptor on the proliferation of vascular smooth muscle cells in rats

作　　者：周子华[1] 廖玉华[1] 王彬[1] 李留东[1] 魏芬[1] 王敏[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,湖北武汉430022

出　　处：《中国病理生理杂志》2006年第2期274-278,共5页Chinese Journal of Pathophysiology

基　　金：973项目资金资助项目(No.G2000056900)

摘　　要：目的:观察抗α1-肾上腺素能受体自身抗体对培养平滑肌细胞增殖的影响,并探讨其可能机制。方法:用酶消化法培养大鼠平滑肌细胞及用亲和层析的方法纯化抗α1-肾上腺素能受体抗体,用BrdU掺入法检测平滑肌细胞的增殖率,用RT-PCR及Western blotting法检测c-jun、c-fos的表达。结果:抗α1-肾上腺素能受体抗体可以显著增加VSMC的增殖,并随时间的延长及抗体滴度的增加而增强,并可增加c-jun mRNA和蛋白的表达,其作用与NE相似,均显著高于正常对照IgG,并均可被α1-肾上腺素能受体阻滞剂prazosin阻断;而该抗体对c-fos的表达无显著增加。结论:抗α1-肾上腺素能受体抗体可以显著增加培养大鼠平滑肌细胞的增殖,并可增加c-jun的表达,在高血压血管重塑中可能发挥一定作用。AIM： The autoantibodies against α1 - adrenergic receptor that was found in patients with malignant hypertension, primary hypertension and refractory hypertension has the agonist activity liked the NE, and may play a role in hypertension. In this paper, the effects of this antibody on vascular smooth muscle cell （VSMC） proliferation and its mechanism were to be studied. METHODS： The cultured rat VSMC proliferation induced by the antibodies against α1 - adrenergic receptor that was purified by the immune affinity chromatography, was measured by the BrdU cell proliferation assay and cell cycle distribution. The expression of c - jun and c - fos were determined by RT - PCR and Western blotting. RESULTS： Compared to the normal IgG, the antibodies against α1 - adrenergic receptor promoted the VSMC proliferation and increased the mRNA and protein expression of the c - jun significantly. The role was similar to the norepinephrine, and all was blocked by prazosin, while the mRNA and protein expression of c - fos were not affected by the antibodies. CONCLUSION： The antibodies against α1 - adrenergic receptor promote the rat VSMC proliferation, and increase the expression of c- jun, which maybe play a role in the vascular remodeling in hypertension.

关 键 词：抗体 受体 肾上腺素能α 细胞增殖 肌 平滑 血管 蛋白质c—jun 蛋白质c—fos 

分 类 号：R363[医药卫生—病理学]