三氧化二砷抑制体外培养小鼠气道成纤维细胞增殖及c-myc与c-sis表达  

Inhibitory effect of arsenic trioxide on the proliferation of airway fibroblast and the expression of c-myc and c-sis in vitro

作　　者：郭红荣[1] 梁标[2] 

机构地区：[1]南京医科大学附属常州第二人民医院呼吸科,江苏常州213003 [2]广东医学院呼吸疾病研究所,广东湛江524021

出　　处：《中国病理生理杂志》2006年第2期314-317,共4页Chinese Journal of Pathophysiology

基　　金：广东省中医药局课题(No.100023)

摘　　要：目的:探讨三氧化二砷(As2O3))对体外培养的小鼠气道成纤维细胞(FB)增殖及原癌基因c-myc与c-sis表达的影响。方法:按不同药物分7组,在体外培养的FB中分别加入浓度为0·25、0·5、1·0、2·0和4·0μmol/L的As2O3,在1、3、5和7d后用记数法观察FB生长情况,并用流式细胞仪(FCM)检测不同浓度药物对各组FB增殖的影响及各组c-myc与c-sis的阳性表达率。结果:各种实验浓度的As2O3对FB均有抑制作用,并有时间剂量效应。流式细胞仪分析显示,随着浓度的增高,G1期细胞比例逐渐增高,G2/M期细胞比例降低,浓度为2·0和4·0μmol/L的As2O3能显著抑制c-myc与c-sis的表达。结论:As2O3能抑制FB的增生,其机理可能与其下调c-myc与c-sis的表达有关。AIM： To study the effects of arsenic trioxide （As203） on mouse airway fibroblast （FB） proliferation and expression of c - myc and c - sis in cultured FB. METHODS： The cultured FB was divided into seven groups. NS, dexamethasone （ 1.0 μmol/L） and As2O3 at concentrations of 0.25, 0.5, 1.0, 2.0, 4.0 μmol/L were instilled respectively into cultured FB. The effects of different concentration of As2O3 and dexamethasone on cell growth were observed at 1, 3, 5 and 7 day. The changes of cell cycle and the positive expression rate of c - myc and c - sis were examined by flow cytometry （FCM）. RESULTS： Various concentrations of As2O3 significantly inhibited the proliferation of FB in vitro and showed a dose - dependent and time - dependent tendency. Data from FCM indicated that Gl - phase cell percentage increased and G2/M - phase cell percentage decreased with the increase in As2O3 concentrations. The expression of c - myc and c - sis was significantly inhibited by As203（2.0, 4.0 μmol/L）. CONCLUSION： As2O3 inhibits FB proliferation by downregulating the expression of c - myc and c - sis.

关 键 词：三氧化二砷 成纤维细胞 细胞增殖 癌基因 

分 类 号：R363[医药卫生—病理学]