CD73对人乳腺癌细胞侵袭力和运动力的影响  

Crucial role for ecto-5'-nucleotidase (CD73) in invasion and migration of human breast cancer cell lines

作　　者：周婷婷[1] 周平[1] 殷莲华[1] 

机构地区：[1]复旦大学上海医学院生理与病理生理学系,上海200032

出　　处：《中国病理生理杂志》2006年第2期360-364,共5页Chinese Journal of Pathophysiology

基　　金：国家"211工程"重点学科建设基金资助项目;教育部回国人员科研基金资助项目

摘　　要：目的:探讨CD73在乳腺癌细胞中的表达特征及其与乳腺癌细胞侵袭转移的相关性。方法:①采用半定量RT-PCR法和高压液相层析法检测6种乳腺癌细胞株CD73的mRNA表达及CD73的活性。②构建CD73真核表达载体pcDNA-NT5E,采用脂质体转染法将其导入无侵袭力、CD73低表达的乳腺癌细胞T47D,同时以空载体质粒pcDNA和未转染细胞作为对照。利用Transwell体外侵袭力实验和运动力实验,观察转染pcDNA-NT5E对T47D细胞侵袭力和运动力的影响。结果:①高度恶性乳腺癌细胞的CD73mRNA水平和CD73活性均显著高于低度恶性细胞(P<0·05)。②转染后细胞T47D的CD73基因转录、CD73酶活性、细胞的侵袭力和运动力明显高于转染前。结论:乳腺癌细胞的恶性程度与CD73表达呈正相关。CD73可增强人乳腺癌细胞的侵袭力和运动力。提示在乳腺癌转移过程中CD73发挥重要的促进作用。AIM： To understand the expression of ecto - 5＇ - nucleotidase （CD73） and explore its effect on the motility and invasion ability of breast cancer cell lines. METHODS： ① CD73 mRNA expression was analyzed by semiquantitative RTPCR. Surface CD73 activity in breast carcinoma cell lines was measured by HPLC according to the conversion of E - AMP to EADO. ② Plasmid pcDNA - NT5E was transfected into human low metastatic breast cancer cell line T47D with lipofectinamine2000. CD73 mRNA expression was detected by semiquantitative RT- PCR and surface CD73 activity was determined by HPLC before and after transfection. The effect of pcDNA - NTSE transfection on motility and invasion ability was observed by in vitro invasion assay. RESULTS： ① The RNA expression and enzyme activity of CD73 were variable in different breast cancer cell lines.② The transfect- T47D overexpressed CD73 at mRNA level and with high enzyme activity. Constitutively active CD73 drove cells transmembrane and invasion into matrigel. Promotion effects were statistically significant （ P 〈 0.05） between pcDNA - NTSE - T47D cell and negative control. CONCLUSION： The CD73 mRNA expression and its enzyme activity in a panel of breast cancer cell lines differ in invasive and metastatic potential. CD73 increases the motility and invasion abilities sharply in the pcDNA - NTSE - T47D cells.

关 键 词：5’-核苷酸酶 腺苷 乳腺肿瘤 肿瘤侵袭 细胞运动 

分 类 号：R363[医药卫生—病理学]