γ-干扰素加强TRAIL抑制人大肠癌细胞株作用的研究  

IFN-γ reinforces the inhibitory effect of tumor necrosis factor-related apoptosis-inducing ligand on apoptosis of human colon cancer cell line RKO cells

作　　者：何超[1] 胡文献[1] 胡晓彤[1] 黄学锋[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院肛肠外科,浙江杭州310016

出　　处：《中国病理生理杂志》2006年第2期365-367,共3页Chinese Journal of Pathophysiology

基　　金：浙江省自然科学基金资助项目(No.302678)

摘　　要：目的:探讨γ-干扰素(IFN-γ)对肿瘤坏死因子相关凋亡诱导配体(TRAIL)抑制人大肠癌细胞株作用的影响。方法:以大肠癌细胞株RKO为对象,采用MTT比色法和流式细胞术研究IFN-γ对TRAIL抑制RKO的影响。结果:IFN-γ组、TRAIL组和IFN-γ72h+TRAIL组的存活分数分别为99·28%、85·45%、52·60%。IFN-γ组、TRAIL组、IFN-γ24h+TRAIL组、IFN-γ48h+TRAIL组和IFN-γ72h+TRAIL组的凋亡率分别为1·51%、2·38%、4·97%、13·30%、21·00%。IFN-γ24h+TRAIL组的凋亡率高于IFN-γ组与TRAIL组的凋亡率之和(P<0·01)。随着IFN-γ预处理时间的延长,TRAIL引起RKO的凋亡率也明显上升(P<0·01)。结论:IFN-γ能有效加强TRAIL对RKO细胞株的凋亡诱导作用。AIM： To explore the influence of IFN - γ on the role of tumor necrosis factor - related apoptosis - inducing ligand （TRAIL） in inducing apoptosis of RKO cell line. METHODS： Survival fraction and apoptosis were measured by MTI＇ method and flow cytometry （ FACS）. RESULTS： Survival fraction of IFN - γ group, TRAIL and IFN - γ 72 h ＋ TRAIL group were 99.28%, 85.45%, 52.60%, respectively. The percentage of apoptotic cells of IFN - γ group, TRAIL group, IFN - γ 24 h ＋ TRAIL group, IFN - γ 48 h ＋ TRAIL and IFN - γ 72 h ＋ TRAIL group were 1.51%, 2.38%, 4.97%, 13.30%, 21.00%, re- spectively. The percentage of apoptotic cells of IFN - γ 24 h ＋ TRAIL group was higher than the sum of IFN - γ group and TRAIL group （ P 〈 0.01 ）. The longer the IFN - γ pretreated, the higher the percentage of apoptotic cells was observed （ P 〈 0.01 ）. CONCLUSION ： IFN - γ can reinforce the effect of TRAIL inducing apoptosis in RKO.

关 键 词：干扰素Ⅱ型 RKO细胞株 肿瘤坏死因子相关凋亡诱导配体 

分 类 号：R363[医药卫生—病理学]