^(131)I标记抗CEA、TPS及混合单抗在荷人乳腺癌裸鼠体内放射免疫显像及生物学分布  

Radioimmunolocalization and blodistribution of ^(131)I labeled anti-CEA,TPS and mixture monoclonal antibody in nude mice bearing human breast cancer xenografts

作　　者：常伟勤[1] 刘元元[2] 许天敏[1] 

机构地区：[1]吉林大学第二医院肿瘤外科,吉林长春130021 [2]吉林大学第一医院传染科,吉林长春130021

出　　处：《哈尔滨医科大学学报》2006年第1期17-19,22,共4页Journal of Harbin Medical University

基　　金：长春市科技计划基金项目(10-082S06)

摘　　要：目的研究131I标记抗CEA、TPS单抗及混合单抗(CEA+TPS)在荷人乳腺癌裸鼠体内的分布,为放免显像(RII)及临床应用提供依据。方法荷瘤鼠分为3组,每组尾静脉分别注入131I标记的抗CEA,TPS及混合单抗3.7MBq/0.1ml,于注射后24、48、961、20h行SPECT,于48、96、120h分批处死,测定肿瘤和血、肝、肺等重要脏器的单位放射性比值(T/NT)。结果标记单抗注射后24~120h内,肿瘤部位出现放射性浓聚,T/NT比值120h最高达21.31。SPECT阳性率分别为50%、58%、91%。结论混合单抗组在T/NT比值、SPECT阳性率方面明显优于对照组。单抗的混合使用有望成为RII的首选方法。Objective To study the distribution of ^131I labeled anti-CEA, TPS and mixture monoclonal antibody （McAb） in nude mice beating human breast cancer and to provide the basis for RⅡ and clinical use.Methods The nude mice were divided into 3 groups randomly, 3.7MBq/0. 1ml labeled anti-CEA, TPS and mixture McAb Was injected into the tall vein each group respectively,the RⅡ were performed at 24,48,96,120 hours by SPECT. Radioactivity ratio of tumor and non-tumor （T/NT）were measured after 48,96,120h,interval of injection. Results Radioactivity concentration in tumor was found during the 24 to 120 hours after injection, the peak value of T/NT was 21.31 at 120 hour. The positive rates of RII were 50 %, 58 %, 91% respectively. Conclusion Mixture McAb group is higher than the control group in the positive rate of SPECT, the value of T/ NT. The monoclonal antibody was used by mixture prospective to be used as the first selection in RⅡ.

关 键 词：乳腺癌 混合单克隆抗体 放射免疫显像 

分 类 号：R737.9[医药卫生—肿瘤] R817.4[医药卫生—临床医学]